Phospholipase D (PLD)
PLD1; Choline Phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Phospholipase D is an enzyme which is located in the plasma membrane and catalyzes the hydrolysis of phosphatidylcholine to form phosphatidic acid (PA), releasing the soluble choline headgroup into the cytosol. There are two mammalian isoforms of phospholipase D: PLD1 and PLD2. PLD directly interacts with kinases like PKC, ERK, TYK and controls the signalling indicating that PLD is activated by these kinases.
As choline is very abundant in the cell, PLD activity does not significantly affect choline levels, and choline is unlikely to play any role in signalling. Phosphatidic acid is a signal molecule and acts to recruit SK1 to membranes. PA is extremely short lived and is rapidly hydrolysed by the enzyme PA phosphohydrolase to form diacylglycerol (DAG).
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU795Hu01 | Recombinant Phospholipase D (PLD) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU795Hu01 | Monoclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IP. |
| PAU795Hu01 | Polyclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU795Hu01 | CLIA Kit for Phospholipase D (PLD) | CLIA assay for Antigen Detection |
| ELU795Hu01 | ELISA Kit for Phospholipase D (PLD) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU795Mu01 | Recombinant Phospholipase D (PLD) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU795Mu01 | Monoclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IP. |
| PAU795Mu01 | Polyclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU795Mu01 | CLIA Kit for Phospholipase D (PLD) | CLIA assay for Antigen Detection |
| ELU795Mu01 | ELISA Kit for Phospholipase D (PLD) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU795Ra01 | Recombinant Phospholipase D (PLD) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU795Ra01 | Monoclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IP. |
| PAU795Ra01 | Polyclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU795Ra01 | CLIA Kit for Phospholipase D (PLD) | CLIA assay for Antigen Detection |
| ELU795Ra01 | ELISA Kit for Phospholipase D (PLD) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU795Ca01 | Recombinant Phospholipase D (PLD) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU795Ca01 | Monoclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IP. |
| PAU795Ca01 | Polyclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU795Ca01 | CLIA Kit for Phospholipase D (PLD) | CLIA assay for Antigen Detection |
| ELU795Ca01 | ELISA Kit for Phospholipase D (PLD) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU795Po01 | Recombinant Phospholipase D (PLD) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU795Po01 | Monoclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IP. |
| PAU795Po01 | Polyclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU795Po01 | CLIA Kit for Phospholipase D (PLD) | CLIA assay for Antigen Detection |
| ELU795Po01 | ELISA Kit for Phospholipase D (PLD) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU795Bo01 | Recombinant Phospholipase D (PLD) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU795Bo01 | Monoclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IP. |
| PAU795Bo01 | Polyclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU795Bo01 | CLIA Kit for Phospholipase D (PLD) | CLIA assay for Antigen Detection |
| ELU795Bo01 | ELISA Kit for Phospholipase D (PLD) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU795Rb01 | Recombinant Phospholipase D (PLD) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU795Rb01 | Monoclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IP. |
| PAU795Rb01 | Polyclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU795Rb01 | CLIA Kit for Phospholipase D (PLD) | CLIA assay for Antigen Detection |
| ELU795Rb01 | ELISA Kit for Phospholipase D (PLD) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU795Mo01 | Recombinant Phospholipase D (PLD) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU795Mo01 | Monoclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IP. |
| PAU795Mo01 | Polyclonal Antibody to Phospholipase D (PLD) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU795Mo01 | CLIA Kit for Phospholipase D (PLD) | CLIA assay for Antigen Detection |
| ELU795Mo01 | ELISA Kit for Phospholipase D (PLD) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family."
J. Biol. Chem. 270:29640-29643(1995) [PubMed] [Europe PMC] [Abstract]
"Characterization of two alternately spliced forms of phospholipase D1. Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric GTP-binding proteins and protein kinase C-alpha."
J. Biol. Chem. 272:3860-3868(1997) [PubMed] [Europe PMC] [Abstract]
"Characterization of human PLD2 and the analysis of PLD isoform splice variants."
FASEB J. 12:1309-1317(1998) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Cloning and initial characterization of a human phospholipase D2 (hPLD2). ADP-ribosylation factor regulates hPLD2."
J. Biol. Chem. 273:12846-12852(1998) [PubMed] [Europe PMC] [Abstract]
"Interaction of the type Ialpha PIPkinase with phospholipase D: a role for the local generation of phosphatidylinositol 4, 5-bisphosphate in the regulation of PLD2 activity."
EMBO J. 19:5440-5449(2000) [PubMed] [Europe PMC] [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]