Deoxyribonuclease II (DNASEII)
DNase-II; DNase2; DNASE2A; DNASE2B; DRN2; DNL; DNL2; Deoxyribonuclease II Alpha; Acid DNase; Lysosomal DNase II
Deoxyribonuclease II, or Acid DNase, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. As the name implies, it is more effective at acid pHs.
There are several known DNases II, including:DNase II alpha (usually just called DNase II) ;DNase II beta (also called DLAD, or DNase II-Like Acid DNase)The predicted 360-amino acid protein has 3 parts: a 16-amino acid signal peptide, a 91-amino acid propeptide, and a 253-amino acid mature protein region. like other lysosomal enzymes, DNase II is processed by release of a signal peptide followed by proteolytic processing that generates a 2-chain enzyme. Purified DNase II migrates as 2 bands (32 and 12 kD) on SDS-PAGE.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU598Hu01 | Recombinant Deoxyribonuclease II (DNASEII) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU598Hu01 | Monoclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IP. |
| PAU598Hu01 | Polyclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU598Hu01 | CLIA Kit for Deoxyribonuclease II (DNASEII) | CLIA assay for Antigen Detection |
| ELU598Hu01 | ELISA Kit for Deoxyribonuclease II (DNASEII) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU598Mu01 | Recombinant Deoxyribonuclease II (DNASEII) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU598Mu01 | Monoclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IP. |
| PAU598Mu01 | Polyclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU598Mu01 | CLIA Kit for Deoxyribonuclease II (DNASEII) | CLIA assay for Antigen Detection |
| ELU598Mu01 | ELISA Kit for Deoxyribonuclease II (DNASEII) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU598Ra01 | Recombinant Deoxyribonuclease II (DNASEII) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU598Ra01 | Monoclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IP. |
| PAU598Ra01 | Polyclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU598Ra01 | CLIA Kit for Deoxyribonuclease II (DNASEII) | CLIA assay for Antigen Detection |
| ELU598Ra01 | ELISA Kit for Deoxyribonuclease II (DNASEII) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU598Ca01 | Recombinant Deoxyribonuclease II (DNASEII) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU598Ca01 | Monoclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IP. |
| PAU598Ca01 | Polyclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU598Ca01 | CLIA Kit for Deoxyribonuclease II (DNASEII) | CLIA assay for Antigen Detection |
| ELU598Ca01 | ELISA Kit for Deoxyribonuclease II (DNASEII) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU598Po01 | Recombinant Deoxyribonuclease II (DNASEII) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU598Po01 | Monoclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IP. |
| PAU598Po01 | Polyclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU598Po01 | CLIA Kit for Deoxyribonuclease II (DNASEII) | CLIA assay for Antigen Detection |
| ELU598Po01 | ELISA Kit for Deoxyribonuclease II (DNASEII) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU598Bo01 | Recombinant Deoxyribonuclease II (DNASEII) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU598Bo01 | Monoclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IP. |
| PAU598Bo01 | Polyclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU598Bo01 | CLIA Kit for Deoxyribonuclease II (DNASEII) | CLIA assay for Antigen Detection |
| ELU598Bo01 | ELISA Kit for Deoxyribonuclease II (DNASEII) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU598Rb01 | Recombinant Deoxyribonuclease II (DNASEII) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU598Rb01 | Monoclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IP. |
| PAU598Rb01 | Polyclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU598Rb01 | CLIA Kit for Deoxyribonuclease II (DNASEII) | CLIA assay for Antigen Detection |
| ELU598Rb01 | ELISA Kit for Deoxyribonuclease II (DNASEII) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPU598Mo01 | Recombinant Deoxyribonuclease II (DNASEII) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAU598Mo01 | Monoclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IP. |
| PAU598Mo01 | Polyclonal Antibody to Deoxyribonuclease II (DNASEII) | WB; IHC; ICC; IF. | |
| Assay Kits | CLU598Mo01 | CLIA Kit for Deoxyribonuclease II (DNASEII) | CLIA assay for Antigen Detection |
| ELU598Mo01 | ELISA Kit for Deoxyribonuclease II (DNASEII) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Structure and organization of the human deoxyribonuclease II (DNase II) gene."
Ann. Hum. Genet. 62:299-305(1998) [PubMed] [Europe PMC] [Abstract]
"Cloning of cDNAs encoding porcine and human DNase II."
Biochem. Biophys. Res. Commun. 247:864-869(1998) [PubMed] [Europe PMC] [Abstract]
"Molecular cloning and characterization of human and murine DNase II."
Gene 215:281-289(1998) [PubMed] [Europe PMC] [Abstract]
"The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis."
J. Biol. Chem. 273:30909-30914(1998) [PubMed] [Europe PMC] [Abstract]
"Molecular cloning of the cDNA encoding human deoxyribonuclease II."
J. Biol. Chem. 273:2610-2616(1998) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"The DNA sequence and biology of human chromosome 19."
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Revised structure of the active form of human deoxyribonuclease IIalpha."
Biochem. Biophys. Res. Commun. 292:415-421(2002) [PubMed] [Europe PMC] [Abstract]
"Structural requirements of human DNase II alpha for formation of the active enzyme: the role of the signal peptide, N-glycosylation, and disulphide bridging."
Biochem. J. 371:867-876(2003) [PubMed] [Europe PMC] [Abstract]
"A family history of deoxyribonuclease II: surprises from Trichinella spiralis and Burkholderia pseudomallei."
Gene 305:1-12(2003) [PubMed] [Europe PMC] [Abstract]
"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]