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Adenine Phosphoribosyltransferase (APRT)

AMP; APRTase

APRTase is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. APRTase is labeled as EC 2.4.2.7. APRT is functionally related to hypoxanthine-guanine phosphoribosyltransferase (HPRT).Deficiency of APRT in human beings may lead to kidney stones formed of adenine and salts.2,8 dihydroxy-adenine urolithiasis is also known as "adenine phosphoribosyltransferase deficiency".

Defects in APRT are the cause of APRT deficiency; also known as 2,8-dihydroxyadenine urolithiasis. It is an autosomal recessive disease characterized by renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU535Hu01 Recombinant Adenine Phosphoribosyltransferase (APRT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU535Hu01 Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IP.
PAU535Hu01 Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IF.
Assay Kits CLU535Hu01 CLIA Kit for Adenine Phosphoribosyltransferase (APRT) CLIA assay for Antigen Detection
ELU535Hu01 ELISA Kit for Adenine Phosphoribosyltransferase (APRT) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU535Mu01 Recombinant Adenine Phosphoribosyltransferase (APRT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU535Mu01 Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IP.
PAU535Mu01 Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IF.
Assay Kits CLU535Mu01 CLIA Kit for Adenine Phosphoribosyltransferase (APRT) CLIA assay for Antigen Detection
ELU535Mu01 ELISA Kit for Adenine Phosphoribosyltransferase (APRT) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU535Ra01 Recombinant Adenine Phosphoribosyltransferase (APRT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU535Ra01 Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IP.
PAU535Ra01 Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IF.
Assay Kits CLU535Ra01 CLIA Kit for Adenine Phosphoribosyltransferase (APRT) CLIA assay for Antigen Detection
ELU535Ra01 ELISA Kit for Adenine Phosphoribosyltransferase (APRT) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU535Ca01 Recombinant Adenine Phosphoribosyltransferase (APRT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU535Ca01 Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IP.
PAU535Ca01 Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IF.
Assay Kits CLU535Ca01 CLIA Kit for Adenine Phosphoribosyltransferase (APRT) CLIA assay for Antigen Detection
ELU535Ca01 ELISA Kit for Adenine Phosphoribosyltransferase (APRT) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU535Po01 Recombinant Adenine Phosphoribosyltransferase (APRT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU535Po01 Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IP.
PAU535Po01 Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IF.
Assay Kits CLU535Po01 CLIA Kit for Adenine Phosphoribosyltransferase (APRT) CLIA assay for Antigen Detection
ELU535Po01 ELISA Kit for Adenine Phosphoribosyltransferase (APRT) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU535Bo01 Recombinant Adenine Phosphoribosyltransferase (APRT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU535Bo01 Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IP.
PAU535Bo01 Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IF.
Assay Kits CLU535Bo01 CLIA Kit for Adenine Phosphoribosyltransferase (APRT) CLIA assay for Antigen Detection
ELU535Bo01 ELISA Kit for Adenine Phosphoribosyltransferase (APRT) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU535Rb01 Recombinant Adenine Phosphoribosyltransferase (APRT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU535Rb01 Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IP.
PAU535Rb01 Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IF.
Assay Kits CLU535Rb01 CLIA Kit for Adenine Phosphoribosyltransferase (APRT) CLIA assay for Antigen Detection
ELU535Rb01 ELISA Kit for Adenine Phosphoribosyltransferase (APRT) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU535Mo01 Recombinant Adenine Phosphoribosyltransferase (APRT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU535Mo01 Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IP.
PAU535Mo01 Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT) WB; IHC; ICC; IF.
Assay Kits CLU535Mo01 CLIA Kit for Adenine Phosphoribosyltransferase (APRT) CLIA assay for Antigen Detection
ELU535Mo01 ELISA Kit for Adenine Phosphoribosyltransferase (APRT) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Nucleotide sequence of the human APRT gene."
Nucleic Acids Res. 15:9086-9086(1987) [PubMed] [Europe PMC] [Abstract]

"Comparative anatomy of the human APRT gene and enzyme: nucleotide sequence divergence and conservation of a nonrandom CpG dinucleotide arrangement."
Proc. Natl. Acad. Sci. U.S.A. 84:3349-3353(1987) [PubMed] [Europe PMC] [Abstract]

"The full-ORF clone resource of the German cDNA consortium."
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

"The sequence and analysis of duplication-rich human chromosome 16."
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Human adenine phosphoribosyltransferase. Complete amino acid sequence of the erythrocyte enzyme."
J. Biol. Chem. 261:13677-13683(1986) [PubMed] [Europe PMC] [Abstract]

"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Three-dimensional structure of human adenine phosphoribosyltransferase and its relation to DHA-urolithiasis."
Biochemistry 43:7663-7671(2004) [PubMed] [Europe PMC] [Abstract]

"Identification of a single missense mutation in the adenine phosphoribosyltransferase (APRT) gene from five Icelandic patients and a British patient."
Am. J. Hum. Genet. 49:1306-1311(1991) [PubMed] [Europe PMC] [Abstract]

"Missense mutation in the adenine phosphoribosyltransferase gene causing 2,8-dihydroxyadenine urolithiasis."
Hum. Mol. Genet. 3:817-818(1994) [PubMed] [Europe PMC] [Abstract]

"Human adenine phosphoribosyltransferase. Identification of allelic mutations at the nucleotide level as a cause of complete deficiency of the enzyme."
J. Clin. Invest. 80:1409-1415(1987) [PubMed] [Europe PMC] [Abstract]

"Human adenine phosphoribosyltransferase deficiency. Demonstration of a single mutant allele common to the Japanese."
J. Clin. Invest. 81:945-950(1988) [PubMed] [Europe PMC] [Abstract]

"Only three mutations account for almost all defective alleles causing adenine phosphoribosyltransferase deficiency in Japanese patients."
J. Clin. Invest. 90:130-135(1992) [PubMed] [Europe PMC] [Abstract]

"2,8-Dihydroxyadenine urolithiasis in a patient with considerable residual adenine phosphoribosyltransferase activity in cell extracts but with mutations in both copies of APRT."
Mol. Genet. Metab. 72:260-264(2001) [PubMed] [Europe PMC] [Abstract]

"Identification of two novel mutations in adenine phosphoribosyltransferase gene in patients with 2,8-dihydroxyadenine urolithiasis."
Nucleosides Nucleotides Nucleic Acids 23:1141-1145(2004) [PubMed] [Europe PMC] [Abstract]

"A Japanese boy with adenine phosphoribosyltransferase (APRT) deficiency caused by compound heterozygosity including a novel missense mutation in APRT gene."
Acta Paediatr. 100:E285-E288(2011) [PubMed] [Europe PMC] [Abstract]