Ninein (NIN)

GSK3B Interacting Protein; Glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein

SPN	U194
HGNC	14906
MGI	105108
PubMed	15458839
RGD	1306492
UniProt	Q8N4C6
Wiki	Ninein

Ninein is one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.The deduced 2,047-amino acid protein has a calculated molecular mass of 239 kD. NIN contains an N-terminal GTP-binding site, a large coiled-coil domain with 4 leucine zipper motifs, and a C-terminal domain responsible for GSK binding. Unlike mouse Nin, it does not have an N-terminal Ca(2+)-binding site. Northern blot analysis detected a 9.0-kb transcript in all tissues examined and a 7.0-kb transcript predominantly in heart and skeletal muscle.

Reference

"Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta."
Biochim. Biophys. Acta 1492:513-516(2000) [PubMed] [Europe PMC] [Abstract]

"Genomic organization and molecular characterization of the human ninein gene."
Biochem. Biophys. Res. Commun. 279:989-995(2000) [PubMed] [Europe PMC] [Abstract]

"Human ninein is a centrosomal autoantigen recognized by CREST patient sera and plays a regulatory role in microtubule nucleation."
Cell Cycle 3:923-930(2004) [PubMed] [Europe PMC] [Abstract]

"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract]

"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and analysis of human chromosome 14."
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation."
J. Cell Sci. 115:1825-1835(2002) [PubMed] [Europe PMC] [Abstract]

"Assembly of centrosomal proteins and microtubule organization depends on PCM-1."
J. Cell Biol. 159:255-266(2002) [PubMed] [Europe PMC] [Abstract]

"Molecular characterization of human ninein protein: two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle."
Biochem. Biophys. Res. Commun. 308:975-983(2003) [PubMed] [Europe PMC] [Abstract]

"A novel ninein-interaction protein, CGI-99, blocks ninein phosphorylation by GSK3beta and is highly expressed in brain tumors."
FEBS Lett. 566:162-168(2004) [PubMed] [Europe PMC] [Abstract]

"Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome."
Arthritis Rheum. 41:551-558(1998) [PubMed] [Europe PMC] [Abstract]

"Spectrum of centrosome autoantibodies in childhood varicella and post-varicella acute cerebellar ataxia."
BMC Pediatr. 3:11-11(2003) [PubMed] [Europe PMC] [Abstract]

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation."
Int. J. Oncol. 37:429-436(2010) [PubMed] [Europe PMC] [Abstract]

"DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome."
Nature 456:66-72(2008) [PubMed] [Europe PMC] [Abstract]

"Novel microcephalic primordial dwarfism disorder associated with variants in the centrosomal protein ninein."
J. Clin. Endocrinol. Metab. 97:E2140-E2151(2012) [PubMed] [Europe PMC] [Abstract]