Centaurin Alpha 1 (CENTa1)

CENT-A1; ADAP1; GCS1L; P42IP4; Arf-GAP with dual PH domain-containing protein 1; Putative MAPK-activating protein PM25

SPN	T929
HGNC	16486
MGI	2442201
PubMed	21057110
RGD	621418
UniProt	O75689
Wiki	CENTA1

By searching an EST database for sequences similar to rat centaurin-alpha, followed by PCR of a blood cDNA library, Venkateswarlu and Cullen cloned CENTA1. The deduced 373-amino acid protein has a calculated molecular mass of about 43 kD. It contains 2 pleckstrin homology (PH) domains and an ARF-GAP homology region. Northern blot analysis detected a 2.5-kb transcript expressed at a high level in brain and at a lower level in peripheral blood leukocytes. By Northern blot analysis, Hanck et al. found that expression of CENTA1, which they called p42-IP4, was high in brain, spinal cord, and peripheral blood, and weak in kidney. No expression was detected in other tissues examined. Fluorescent-labeled CENTA1 localized diffusely in cytosol and nucleus of transfected human embryonic kidney cells.

Reference

"Molecular cloning and functional characterization of a human homologue of centaurin-alpha."
Biochem. Biophys. Res. Commun. 262:237-244(1999) [PubMed] [Europe PMC] [Abstract].

"Molecular identification of a high-affinity Ins(1,3,4,5)tetrakisphosphate/phosphatidylinositol(3,4, 5)trisphosphate binding protein from human brain, p42IP4."
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.

"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Human chromosome 7: DNA sequence and biology."
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract].

"Centaurin-alpha(1) associates with and is phosphorylated by isoforms of protein kinase C."
Biochem. Biophys. Res. Commun. 307:459-465(2003) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of full length centaurin alpha-1."
Submitted (DEC-2008) to the PDB data bank