Aspartyl tRNA Synthetase (DARS)

AspRS; PIG40; Aspartate tRNA Ligase 1,Cytoplasmic; Cell proliferation-inducing gene 40 protein

SPN	T777
HGNC	2678
MGI	2442544
PubMed	8313877
RGD	621167
UniProt	P14868
Wiki	DARS_(gene

DARS charges its cognate tRNA with aspartate during protein biosynthesis.The predicted DARS protein has 500 amino acids and a calculated molecular mass of 57,000 Da. The human DARS protein has 95% amino acid identity with rat Dars and shares unevenly distributed identity with yeast aspartyl-tRNA synthetase, with 69% identity at the C-terminal half and 46% identity at the N-terminal half. The authors discussed several potential functional domains in the DAR protein, including a region of conserved lysine residues which is also found in bacterial and yeast synthetases and is likely to be the binding site for the 3-prime end of tRNA, a nucleotide triphosphate-binding motif, an ATP-binding motif with strong similarity to that present in E. coli alanyl-tRNA synthetase, and a cyclic AMP-dependent protein kinase phosphorylation site.

Reference

"cDNA sequence, predicted primary structure, and evolving amphiphilic helix of human aspartyl-tRNA synthetase."
J. Biol. Chem. 264:16608-16612(1989) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The full-ORF clone resource of the German cDNA consortium."
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Lysyl-tRNA synthetase interacts with EF1alpha, aspartyl-tRNA synthetase and p38 in vitro."
Biochem. Biophys. Res. Commun. 365:718-723(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Mutations in DARS cause hypomyelination with brain stem and spinal cord involvement and leg spasticity."
Am. J. Hum. Genet. 92:774-780(2013) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]