X-Ray Repair Cross Complementing 5 (XRCC5)

KARP1; KU80; KUB2; Ku86; NFIV; TLAA; Lupus Ku Autoantigen Protein P80; Nuclear factor IV; 86 kDa of Ku antigen; CTC box-binding factor 85 kDa; Thyroid-lupus autoantigen

SPN	T772
HGNC	12833
MGI	104517
PubMed	17214517
RGD	3976
UniProt	P13010
Wiki	Ku80

Ku80 is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.Ku was originally identified when patients with systemic lupus erythematosus were found to have high levels of autoantibodies to the protein.

Reference

"cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen."
J. Biol. Chem. 264:13407-13411(1989) [PubMed] [Europe PMC] [Abstract]

"Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome."
Proc. Natl. Acad. Sci. U.S.A. 87:1777-1781(1990) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter."
J. Biol. Chem. 265:17911-17920(1990) [PubMed] [Europe PMC] [Abstract]

"Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen."
EMBO J. 13:4991-5001(1994) [PubMed] [Europe PMC] [Abstract]

"Non-histone protein 1 (NHP1) is a member of the Ku protein family which is upregulated in differentiating mouse myoblasts and human promyelocytes."
FEBS Lett. 382:313-318(1996) [PubMed] [Europe PMC] [Abstract]

"The autoantigen Ku is indistinguishable from NF IV, a protein forming multimeric protein-DNA complexes."
J. Exp. Med. 172:1049-1054(1990) [PubMed] [Europe PMC] [Abstract]

"DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen."
Biochemistry 33:8548-8557(1994) [PubMed] [Europe PMC] [Abstract]

"The interaction between Ku antigen and REF1 protein mediates negative gene regulation by extracellular calcium."
J. Biol. Chem. 271:8593-8598(1996) [PubMed] [Europe PMC] [Abstract]

"Identification of proteins binding to interferon-inducible transcriptional enhancers in hematopoietic cells."
J. Biol. Chem. 267:4533-4540(1992) [PubMed] [Europe PMC] [Abstract]

"Purification of the sequence-specific transcription factor CTCBF, involved in the control of human collagen IV genes: subunits with homology to Ku antigen."
EMBO J. 14:791-800(1995) [PubMed] [Europe PMC] [Abstract]

"DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer."
Biochemistry 38:1819-1828(1999) [PubMed] [Europe PMC] [Abstract]

"Regulation of osteocalcin gene expression by a novel Ku antigen transcription factor complex."
J. Biol. Chem. 277:37280-37291(2002) [PubMed] [Europe PMC] [Abstract]

"Positive and negative modulation of the transcriptional activity of the ETS factor ESE-1 through interaction with p300, CREB-binding protein, and Ku 70/86."
J. Biol. Chem. 279:25241-25250(2004) [PubMed] [Europe PMC] [Abstract]

"Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates."
J. Biol. Chem. 280:5004-5012(2005) [PubMed] [Europe PMC] [Abstract]

"Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage."
Nature 434:605-611(2005) [PubMed] [Europe PMC] [Abstract]

"A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses."
EMBO J. 26:2094-2103(2007) [PubMed] [Europe PMC] [Abstract]

"APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks."
Mol. Cell. Biol. 27:3793-3803(2007) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Ku is a 5'-dRP/AP lyase that excises nucleotide damage near broken ends."
Nature 464:1214-1217(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."
Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]

"Deformed epidermal autoregulatory factor-1 (DEAF1) interacts with the Ku70 subunit of the DNA-dependent protein kinase complex."
PLoS ONE 7:E33404-E33404(2012) [PubMed] [Europe PMC] [Abstract]

"The E3 ligase RNF8 regulates KU80 removal and NHEJ repair."
Nat. Struct. Mol. Biol. 19:201-206(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair."
Science 347:185-188(2015) [PubMed] [Europe PMC] [Abstract]

"Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair."
Nature 412:607-614(2001) [PubMed] [Europe PMC] [Abstract]