Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B)
LOX12B; 12R-LOX; Lipoxygenase, 12R; Epidermis-type lipoxygenase 12
- 12R-lipoxygenase catalyzes the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid (12R-HETE). The ALOX12B cDNA showed the greatest sequence similarity to the second type of human 15S-lipoxygenase (ALOX15B), and was more distantly related to human 12S-lipoxygenase (ALOX12). The 2 are coexpressed in tissues, and either gene can be the site of mutations causing NCIE, indicating a functional relationship. Nonbullous congenital ichthyosiform erythroderma (NCIE) is a nonsyndromic form of autosomal recessive congenital ichthyosis characterized by hyperkeratosis and a disruption in the epidermal permeability barrier. Identification of mutations in two lipoxygenases (LOXs), ALOX12B (12R-LOX) and ALOXE3 (eLOX3), and further functional studies implicate ALOX12B and ALOXE3 in the etiology of NCIE.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPT703Hu01 | Recombinant Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAT703Hu01 | Monoclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IP. |
| PAT703Hu01 | Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IF. | |
| Assay Kits | CLT703Hu01 | CLIA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | CLIA assay for Antigen Detection |
| ELT703Hu01 | ELISA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPT703Mu01 | Recombinant Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAT703Mu01 | Monoclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IP. |
| PAT703Mu01 | Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IF. | |
| Assay Kits | CLT703Mu01 | CLIA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | CLIA assay for Antigen Detection |
| ELT703Mu01 | ELISA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPT703Ra01 | Recombinant Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAT703Ra01 | Monoclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IP. |
| PAT703Ra01 | Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IF. | |
| Assay Kits | CLT703Ra01 | CLIA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | CLIA assay for Antigen Detection |
| ELT703Ra01 | ELISA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPT703Ca01 | Recombinant Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAT703Ca01 | Monoclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IP. |
| PAT703Ca01 | Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IF. | |
| Assay Kits | CLT703Ca01 | CLIA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | CLIA assay for Antigen Detection |
| ELT703Ca01 | ELISA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPT703Po01 | Recombinant Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAT703Po01 | Monoclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IP. |
| PAT703Po01 | Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IF. | |
| Assay Kits | CLT703Po01 | CLIA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | CLIA assay for Antigen Detection |
| ELT703Po01 | ELISA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPT703Bo01 | Recombinant Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAT703Bo01 | Monoclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IP. |
| PAT703Bo01 | Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IF. | |
| Assay Kits | CLT703Bo01 | CLIA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | CLIA assay for Antigen Detection |
| ELT703Bo01 | ELISA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPT703Rb01 | Recombinant Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAT703Rb01 | Monoclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IP. |
| PAT703Rb01 | Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IF. | |
| Assay Kits | CLT703Rb01 | CLIA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | CLIA assay for Antigen Detection |
| ELT703Rb01 | ELISA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPT703Mo01 | Recombinant Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAT703Mo01 | Monoclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IP. |
| PAT703Mo01 | Polyclonal Antibody to Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | WB; IHC; ICC; IF. | |
| Assay Kits | CLT703Mo01 | CLIA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | CLIA assay for Antigen Detection |
| ELT703Mo01 | ELISA Kit for Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"A 12R-lipoxygenase in human skin: mechanistic evidence, molecular cloning, and expression."
Proc. Natl. Acad. Sci. U.S.A. 95:6744-6749(1998) [PubMed] [Europe PMC] [Abstract]
"Human 12(R)-lipoxygenase and the mouse ortholog. Molecular cloning, expression, and gene chromosomal assignment."
J. Biol. Chem. 273:33540-33547(1998) [PubMed] [Europe PMC] [Abstract]
"A gene cluster encoding human epidermis-type lipoxygenases at chromosome 17p13.1: cloning, physical mapping, and expression."
Genomics 73:323-330(2001) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope."
J. Biol. Chem. 286:24046-24056(2011) [PubMed] [Europe PMC] [Abstract]
"A role for 12R-lipoxygenase in MUC5AC expression by respiratory epithelial cells."
Eur. Respir. J. 40:714-723(2012) [PubMed] [Europe PMC] [Abstract]
"Lipoxygenase-3 (ALOXE3) and 12(R)-lipoxygenase (ALOX12B) are mutated in non-bullous congenital ichthyosiform erythroderma (NCIE) linked to chromosome 17p13.1."
Hum. Mol. Genet. 11:107-113(2002) [PubMed] [Europe PMC] [Abstract]
"Mutations associated with a congenital form of ichthyosis (NCIE) inactivate the epidermal lipoxygenases 12R-LOX and eLOX3."
Biochim. Biophys. Acta 1686:238-247(2005) [PubMed] [Europe PMC] [Abstract]
"Mutation spectrum and functional analysis of epidermis-type lipoxygenases in patients with autosomal recessive congenital ichthyosis."
Hum. Mutat. 26:351-361(2005) [PubMed] [Europe PMC] [Abstract]
"Molecular analysis of 250 patients with autosomal recessive congenital ichthyosis: evidence for mutation hotspots in ALOXE3 and allelic heterogeneity in ALOX12B."
J. Invest. Dermatol. 129:1421-1428(2009) [PubMed] [Europe PMC] [Abstract]
"Genotypic and clinical spectrum of self-improving collodion ichthyosis: ALOX12B, ALOXE3, and TGM1 mutations in Scandinavian patients."
J. Invest. Dermatol. 130:438-443(2010) [PubMed] [Europe PMC] [Abstract]