Ribonuclease A2 (RNASE2)

Rnase-A2; Rnase2; EDN; Ribonuclease US; Ribonuclease,RNase A Family,2; Liver,Eosinophil-Derived Neurotoxin; MNon-secretory ribonuclease

SPN	T613
HGNC	10045
MGI	1858238
PubMed	18673278
RGD	1359432
UniProt	P10153
Wiki	RNASE2

Eosinophil-derived neurotoxin is a distinct cationic protein of the eosinophil's large specific granule known primarily for its ability to induce ataxia, paralysis, and central nervous system cellular degeneration in experimental animals (Gordon phenomenon). Rosenberg et al. (1989) isolated a 725-bp cDNA clone for EDN. The open reading frame encodes a 134-amino acid polypeptide with a molecular mass of 15.5 kD and a 27-residue N-terminal hydrophobic leader sequence. The sequence of the mature polypeptide was identical to that reported for human urinary ribonuclease and to the N-terminal sequence of human liver ribonuclease. Similarities to the ribonucleases of pancreas and angiogenin indicate that EDN belongs to the ribonuclease multigene family. This gene is also symbolized RNS2 for ribonuclease 2.

Reference

"Sequence of human eosinophil-derived neurotoxin cDNA: identity of deduced amino acid sequence with human nonsecretory ribonucleases."
Gene 83:161-167(1989) [PubMed] [Europe PMC] [Abstract]

"Eosinophil cationic protein cDNA. Comparison with other toxic cationic proteins and ribonucleases."
J. Immunol. 143:952-955(1989) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning of the human eosinophil-derived neurotoxin: a member of the ribonuclease gene family."
Proc. Natl. Acad. Sci. U.S.A. 86:4460-4464(1989) [PubMed] [Europe PMC] [Abstract]

"Structure and chromosome localization of the human eosinophil-derived neurotoxin and eosinophil cationic protein genes: evidence for intronless coding sequences in the ribonuclease gene superfamily."
Genomics 7:535-546(1990) [PubMed] [Europe PMC] [Abstract]

"Sequence variation at two eosinophil-associated ribonuclease loci in humans."
Genetics 156:1949-1958(2000) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Amino acid sequence of the nonsecretory ribonuclease of human urine."
Biochemistry 27:4530-4538(1988) [PubMed] [Europe PMC] [Abstract]

"Biochemical and functional similarities between human eosinophil-derived neurotoxin and eosinophil cationic protein: homology with ribonuclease."
Proc. Natl. Acad. Sci. U.S.A. 83:3146-3150(1986) [PubMed] [Europe PMC] [Abstract]

"Purification and properties of bovine kidney ribonucleases."
J. Biochem. 97:923-934(1985) [PubMed] [Europe PMC] [Abstract]

"Purification and characterization of a ribonuclease from human liver."
J. Biol. Chem. 263:16125-16131(1988) [PubMed] [Europe PMC] [Abstract]

"Characterization of a unique nonsecretory ribonuclease from urine of pregnant women."
J. Biochem. 111:325-330(1992) [PubMed] [Europe PMC] [Abstract]

"Characterisation of UGP and its relationship with beta-core fragment."
Br. J. Cancer 67:686-692(1993) [PubMed] [Europe PMC] [Abstract]

"New type of linkage between a carbohydrate and a protein: C-glycosylation of a specific tryptophan residue in human RNase Us."
Biochemistry 33:13524-13530(1994) [PubMed] [Europe PMC] [Abstract]

"The hexopyranosyl residue that is C-glycosidically linked to the side chain of tryptophan-7 in human RNase Us is alpha-mannopyranose."
Biochemistry 34:11785-11789(1995) [PubMed] [Europe PMC] [Abstract]

"Recognition signal for C-mannosylation of Trp-7 in RNase 2 consists of sequence Trp-x-x-Trp."
Mol. Biol. Cell 9:301-309(1998) [PubMed] [Europe PMC] [Abstract]

"Mutational analysis of the complex of human RNase inhibitor and human eosinophil-derived neurotoxin (RNase 2)."
Biochemistry 42:1451-1459(2003) [PubMed] [Europe PMC] [Abstract]

"Eosinophil-derived neurotoxin (EDN), an antimicrobial protein with chemotactic activities for dendritic cells."
Blood 102:3396-3403(2003) [PubMed] [Europe PMC] [Abstract]

"Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase."
J. Biol. Chem. 283:28629-28640(2008) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"X-ray crystallographic structure of recombinant eosinophil-derived neurotoxin at 1.83-A resolution."
J. Mol. Biol. 260:540-552(1996) [PubMed] [Europe PMC] [Abstract]

"Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors."
J. Biol. Chem. 276:15009-15017(2001) [PubMed] [Europe PMC] [Abstract]

"Molecular recognition of human eosinophil-derived neurotoxin (RNase 2) by placental ribonuclease inhibitor."
J. Mol. Biol. 347:637-655(2005) [PubMed] [Europe PMC] [Abstract]

"Crystal structures of eosinophil-derived neurotoxin (EDN) in complex with the inhibitors 5'-ATP, Ap3A, Ap4A, and Ap5A."
Biochemistry 45:416-426(2006) [PubMed] [Europe PMC] [Abstract]