Complement Component 8a (C8a)

Complement Component 8,Alpha Polypeptide; Complement Component 8 Deficiency,Type I

SPN	T001
HGNC	1352
MGI	2668347
PubMed	2759635
RGD	1308355
UniProt	P07357
Wiki	C8A

In a 24-year-old black woman with 3 episodes of disseminated gonococcal infection, Petersen et al. found severe deficiency of C8. Michelotti et al. isolated overlapping genomic clones and used them to decipher the organization of the human C8A gene. The gene contains at least 11 exons and spans approximately 70 kb of DNA. C8A genomic organization was found to be remarkably similar to that of C6, C8B, and C9. Although the C8A and C8B loci were previously reported to be less than 2.5-kb apart, Michelotti et al. obtained results using exon-specific probes, indicating that the loci are not as closely linked as initially believed. Komatsu et al. and Komatsu et al. described hereditary C8-alpha-gamma deficiency in the rabbit where it was associated with dwarfism, small thymus, small litter size, and low survival rate.

Reference

"Complementary DNA and derived amino acid sequence of the alpha subunit of human complement protein C8: evidence for the existence of a separate alpha subunit messenger RNA."
Biochemistry 26:3556-3564(1987) [PubMed] [Europe PMC] [Abstract]

"Genomic organization of human complement protein C8 alpha and further examination of its linkage to C8 beta."
Hum. Genet. 95:513-518(1995) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The eighth component of human complement. Purification and physicochemical characterization of its unusual subunit structure."
J. Biol. Chem. 255:11997-12005(1980) [PubMed] [Europe PMC] [Abstract]

"The four terminal components of the complement system are C-mannosylated on multiple tryptophan residues."
J. Biol. Chem. 274:32786-32794(1999) [PubMed] [Europe PMC] [Abstract]

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Structure of C8alpha-MACPF reveals mechanism of membrane attack in complement immune defense."
Science 317:1552-1554(2007) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the MACPF domain of human complement protein C8 alpha in complex with the C8 gamma subunit."
J. Mol. Biol. 379:331-342(2008) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of complement protein C8gamma in complex with a peptide containing the C8gamma binding site on C8alpha: implications for C8gamma ligand binding."
Mol. Immunol. 45:750-756(2008) [PubMed] [Europe PMC] [Abstract]

"The eighth component of human complement: molecular basis of C8A (C81) polymorphism."
Hum. Genet. 96:281-284(1995) [PubMed] [Europe PMC] [Abstract]