5',3'-Nucleotidase, Cytosolic (NT5C)

DNT; DNT1; P5N2; PN-I; PN-II; UMPH2; cdN; 5'Nucleotidase,Deoxy(Pyrimidine)Cytosolic Type C; Uridine 5-Prime Monophosphate Hydrolase 2; 5'(3')-deoxyribonucleotidase, cytosolic

SPN	S851
HGNC	17144
MGI	1354954
PubMed	21873433
UniProt	Q8TCD5
Wiki	NT5C

Pyrimidine 5-prime nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2).

Detected in skeletal muscle, heart and pancreas.

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS851Hu01	Recombinant 5',3'-Nucleotidase, Cytosolic (NT5C)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS851Hu01	Monoclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IP.
Antibodies	PAS851Hu01	Polyclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IF.
Assay Kits	CLS851Hu01	CLIA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	CLIA assay for Antigen Detection
Assay Kits	ELS851Hu01	ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS851Mu01	Recombinant 5',3'-Nucleotidase, Cytosolic (NT5C)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS851Mu01	Monoclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IP.
Antibodies	PAS851Mu01	Polyclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IF.
Assay Kits	CLS851Mu01	CLIA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	CLIA assay for Antigen Detection
Assay Kits	ELS851Mu01	ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS851Ra01	Recombinant 5',3'-Nucleotidase, Cytosolic (NT5C)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS851Ra01	Monoclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IP.
Antibodies	PAS851Ra01	Polyclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IF.
Assay Kits	CLS851Ra01	CLIA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	CLIA assay for Antigen Detection
Assay Kits	ELS851Ra01	ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS851Ca01	Recombinant 5',3'-Nucleotidase, Cytosolic (NT5C)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS851Ca01	Monoclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IP.
Antibodies	PAS851Ca01	Polyclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IF.
Assay Kits	CLS851Ca01	CLIA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	CLIA assay for Antigen Detection
Assay Kits	ELS851Ca01	ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS851Po01	Recombinant 5',3'-Nucleotidase, Cytosolic (NT5C)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS851Po01	Monoclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IP.
Antibodies	PAS851Po01	Polyclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IF.
Assay Kits	CLS851Po01	CLIA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	CLIA assay for Antigen Detection
Assay Kits	ELS851Po01	ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS851Bo01	Recombinant 5',3'-Nucleotidase, Cytosolic (NT5C)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS851Bo01	Monoclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IP.
Antibodies	PAS851Bo01	Polyclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IF.
Assay Kits	CLS851Bo01	CLIA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	CLIA assay for Antigen Detection
Assay Kits	ELS851Bo01	ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS851Rb01	Recombinant 5',3'-Nucleotidase, Cytosolic (NT5C)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS851Rb01	Monoclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IP.
Antibodies	PAS851Rb01	Polyclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IF.
Assay Kits	CLS851Rb01	CLIA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	CLIA assay for Antigen Detection
Assay Kits	ELS851Rb01	ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS851Mo01	Recombinant 5',3'-Nucleotidase, Cytosolic (NT5C)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS851Mo01	Monoclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IP.
Antibodies	PAS851Mo01	Polyclonal Antibody to 5',3'-Nucleotidase, Cytosolic (NT5C)	WB; IHC; ICC; IF.
Assay Kits	CLS851Mo01	CLIA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	CLIA assay for Antigen Detection
Assay Kits	ELS851Mo01	ELISA Kit for 5',3'-Nucleotidase, Cytosolic (NT5C)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells."
J. Biol. Chem. 275:5409-5415(2000) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Mouse cytosolic and mitochondrial deoxyribonucleotidases: cDNA cloning of the mitochondrial enzyme, gene structures, chromosomal mapping and comparison with the human orthologs."
Gene 294:109-117(2002) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Crystal structures of human and murine deoxyribonucleotidases: insights into recognition of substrates and nucleotide analogues."
Biochemistry 46:13809-13818(2007) [PubMed] [Europe PMC] [Abstract]