Liver Receptor Homolog 1 (LRH1)

NR5A2; B1F; B1F2; CPF; FTF; FTZ-F1; LRH-1; hB1F; hB1F-2; Hepatocytic transcription factor; Alpha-1-fetoprotein transcription factor; Nuclear Receptor Subfamily 5,Group A,Member 2

SPN	S824
HGNC	7984
MGI	1346834
RGD	68353
UniProt	O00482

By means of yeast 1-hybrid screening of a liver cDNA library, Li et al. (1998) cloned a cDNA encoding a novel hepatocyte transcription factor, which they called HB1F for human B1-binding factor. The deduced 495-amino acid protein, which has a molecular mass of 54 kD, belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors and is closely related to steroidogenic factor-1 (SF1), another member of this subfamily.

HB1F contains a DNA-binding domain with 2 zinc finger motifs, an FTZ-F1 box, and a ligand-binding domain. Northern blot analysis revealed that HB1F is expressed in liver, pancreas, and lung as a 5.2-kb transcript. An additional transcript of 3.8 kb was present in hepatoma cells HepG2. The authors identified 2 HB1F isoforms which differ in their A/B region.

Reference

"Cloning and characterization of a novel human hepatocyte transcription factor, hB1F, which binds and activates enhancer II of hepatitis B virus."
J. Biol. Chem. 273:29022-29031(1998) [PubMed] [Europe PMC] [Abstract]

"CPF: an orphan nuclear receptor that regulates liver-specific expression of the human cholesterol 7alpha-hydroxylase gene."
Proc. Natl. Acad. Sci. U.S.A. 96:6660-6665(1999) [PubMed] [Europe PMC] [Abstract]

"Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene."
Gene 273:239-249(2001) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Assignment of the fetoprotein transcription factor gene (FTF) to human chromosome band 1q32.11 by in situ hybridization."
Cytogenet. Cell Genet. 82:269-270(1998) [PubMed] [Europe PMC] [Abstract]

"Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1."
Cell 120:343-355(2005) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the human LRH-1 DBD-DNA complex reveals Ftz-F1 domain positioning is required for receptor activity."
J. Mol. Biol. 354:1091-1102(2005) [PubMed] [Europe PMC] [Abstract]

"Modulation of human nuclear receptor LRH-1 activity by phospholipids and SHP."
Nat. Struct. Mol. Biol. 12:357-363(2005) [PubMed] [Europe PMC] [Abstract]

"The crystal structures of human steroidogenic factor-1 and liver receptor homologue-1."
Proc. Natl. Acad. Sci. U.S.A. 102:7505-7510(2005) [PubMed] [Europe PMC] [Abstract]