4-Hydroxyphenylpyruvate Dioxygenase (HPD)

PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase

SPN	S534
HGNC	5147
MGI	96213
PubMed	15581571
RGD	61974
UniProt	P32754
Wiki	HPD

4-Hydroxyphenylpyruvate dioxygenase HPPD is an Fe-containing enzyme, that catalyzes the second reaction in the catabolism of tyrosine - the conversion of 4-hydroxyphenylpyruvate to homogentisate.The mechanism of action of 4-hydroxyphenylpyruvate dioxygenase involves a decarboxylation step and an oxidation step, followed by a rearrangement step to form homogentisic acid. The HPPD reaction occurs through a NIH shift and involves the oxidative decarboxylation of an α-oxo acid as well as aromatic ring hydroxylation. The NIH-shift, which has been demonstrated through isotope-labeling studies, involves migration of an alkyl group to form a more stable carbocation. The shift, accounts for the observation that C3 is bonded to C4 in 4-hydroxyphenylpyruvate but to C5 in homogentisate.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS534Hu01	Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS534Hu01	Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IP.
Antibodies	PAS534Hu01	Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IF.
Assay Kits	CLS534Hu01	CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	CLIA assay for Antigen Detection
Assay Kits	ELS534Hu01	ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS534Mu01	Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS534Mu01	Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IP.
Antibodies	PAS534Mu01	Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IF.
Assay Kits	CLS534Mu01	CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	CLIA assay for Antigen Detection
Assay Kits	ELS534Mu01	ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS534Ra01	Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS534Ra01	Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IP.
Antibodies	PAS534Ra01	Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IF.
Assay Kits	CLS534Ra01	CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	CLIA assay for Antigen Detection
Assay Kits	ELS534Ra01	ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS534Ca01	Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS534Ca01	Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IP.
Antibodies	PAS534Ca01	Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IF.
Assay Kits	CLS534Ca01	CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	CLIA assay for Antigen Detection
Assay Kits	ELS534Ca01	ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS534Po01	Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS534Po01	Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IP.
Antibodies	PAS534Po01	Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IF.
Assay Kits	CLS534Po01	CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	CLIA assay for Antigen Detection
Assay Kits	ELS534Po01	ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS534Bo01	Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS534Bo01	Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IP.
Antibodies	PAS534Bo01	Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IF.
Assay Kits	CLS534Bo01	CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	CLIA assay for Antigen Detection
Assay Kits	ELS534Bo01	ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS534Rb01	Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS534Rb01	Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IP.
Antibodies	PAS534Rb01	Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IF.
Assay Kits	CLS534Rb01	CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	CLIA assay for Antigen Detection
Assay Kits	ELS534Rb01	ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPS534Mo01	Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAS534Mo01	Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IP.
Antibodies	PAS534Mo01	Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	WB; IHC; ICC; IF.
Assay Kits	CLS534Mo01	CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	CLIA assay for Antigen Detection
Assay Kits	ELS534Mo01	ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Structure of the human 4-hydroxyphenylpyruvic acid dioxygenase gene (HPD)."
Genomics 23:534-539(1994) [PubMed] [Europe PMC] [Abstract]

"Regional assignment of the human 4-hydroxyphenylpyruvate dioxygenase gene (HPD) to 12q24-->qter by fluorescence in situ hybridization."
Cytogenet. Cell Genet. 71:374-376(1995) [PubMed] [Europe PMC] [Abstract]

"Human 4-hydroxyphenylpyruvate dioxygenase gene (HPD)."
Genomics 44:292-299(1997) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The finished DNA sequence of human chromosome 12."
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human 4-hydroxyphenylpyruvate dioxygenase."
Submitted (SEP-2009) to the PDB data bank

"Mutations in the 4-hydroxyphenylpyruvate dioxygenase gene (HPD) in patients with tyrosinemia type III."
Hum. Genet. 106:654-662(2000) [PubMed] [Europe PMC] [Abstract]

"Mutations in the 4-hydroxyphenylpyruvic acid dioxygenase gene are responsible for tyrosinemia type III and hawkinsinuria."
Mol. Genet. Metab. 71:506-510(2000) [PubMed] [Europe PMC] [Abstract]