Rac-GTPase Activating Protein 1 (RACGAP1)

HsCYK-4; ID-GAP; MgcRacGAP; Male germ cell RacGap

SPN	S487
HGNC	9804
MGI	1349423
PubMed	24019536
RGD	1305912
UniProt	Q9H0H5
Wiki	RACGAP1

The predicted 527-amino acid RACGAP1 protein has a large N-terminal region containing a protein kinase C -like cysteine-rich motif. The authors determined that RACGAP1 shares highest homology with the Drosophila RnRacGAP and the chimerins of rat and human. Functional analysis showed that the GAP domain of RACGAP1 exhibits strong GAP activity towards CDC42, RAC1, and RAC2. Northern blot analysis detected an approximately 3.2-kb RACGAP1 transcript that was most abundantly expressed in testis, with low expression in most other tissues. Western blot analysis detected a RACGAP1 protein of 58 kD in testis extracts. In situ hybridization showed that RACGAP1 expression is restricted to germ cells in mature testis.

Reference

"MgcRacGAP is involved in the control of growth and differentiation of hematopoietic cells."
Blood 96:2116-2124(2000) [PubMed] [Europe PMC] [Abstract]

"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract].

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] and Testis.

"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]

Ref.6"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]

"MgcRacGAP, a new human GTPase-activating protein for Rac and Cdc42 similar to Drosophila rotundRacGAP gene product, is expressed in male germ cells."
J. Biol. Chem. 273:6019-6023(1998) [PubMed] [Europe PMC] [Abstract]

"MgcRacGAP is involved in cytokinesis through associating with mitotic spindle and midbody."
J. Biol. Chem. 276:5821-5828(2001) [PubMed] [Europe PMC] [Abstract]

"Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling."
J. Biol. Chem. 276:20309-20315(2001) [PubMed] [Europe PMC] [Abstract]

"Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity."
Dev. Cell 2:41-54(2002) [PubMed] [Europe PMC] [Abstract]

"Rho family GTPase Rnd2 interacts and co-localizes with MgcRacGAP in male germ cells."
Biochem. J. 372:105-112(2003) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation by aurora B converts MgcRacGAP to a RhoGAP during cytokinesis."
Dev. Cell 4:549-560(2003) [PubMed] [Europe PMC] [Abstract]

"MgcRacGAP regulates cortical activity through RhoA during cytokinesis."
Exp. Cell Res. 293:275-282(2004) [PubMed] [Europe PMC] [Abstract]

"Human mitotic spindle-associated protein PRC1 inhibits MgcRacGAP activity toward Cdc42 during the metaphase."
J. Biol. Chem. 279:16394-16402(2004) [PubMed] [Europe PMC] [Abstract]

"Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis."
J. Cell Biol. 168:221-232(2005) [PubMed] [Europe PMC] [Abstract]

"An ECT2-centralspindlin complex regulates the localization and function of RhoA."
J. Cell Biol. 170:571-582(2005) [PubMed] [Europe PMC] [Abstract]

"MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis."
Proc. Natl. Acad. Sci. U.S.A. 102:13158-13163(2005) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"Dissecting the role of Rho-mediated signaling in contractile ring formation."
Mol. Biol. Cell 17:43-55(2006) [PubMed] [Europe PMC] [Abstract]

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

"Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis."
EMBO J. 27:1791-1803(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells."
PLoS Biol. 7:E1000111-E1000111(2009) [PubMed] [Europe PMC] [Abstract]

"Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation."
PLoS Biol. 7:E1000110-E1000110(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis."
Nature 492:276-279(2012) [PubMed] [Europe PMC] [Abstract]