Ubiquitin Conjugating Enzyme E2S (UBE2S)
E2-EPF; Ubiquitin Carrier Protein; Ubiquitin-Protein Ligase; Ubiquitin-protein ligase S
- The ubiquitin-proteasome protein degradation pathway involves 3 enzymes: E1, which forms a thioester bond with ubiquitin; E2, which transiently carries the activated ubiquitin molecule as a thioester; and E3, which transfers the activated ubiquitin from the E2 to the substrate.
- UBE2S behaves as an E2 ubiquitin carrier protein.The longest open reading frame (EPF5-ORF2) encodes a deduced 225-amino acid protein with a calculated molecular mass of 24 kD. It shares 38% identity with yeast Ubc4 and is highly similar to ubiquitin carrier proteins in the core region containing the active-site cysteine. Western blot analysis revealed that EPF serum recognized a much shorter protein encoded by an alternate reading frame, EPF5-ORF1.
Organism species: Homo sapiens (Human)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPS190Hu01 | Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAS190Hu01 | Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IP. |
PAS190Hu01 | Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IF. | |
Assay Kits | CLS190Hu01 | CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | CLIA assay for Antigen Detection |
ELS190Hu01 | ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPS190Mu01 | Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAS190Mu01 | Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IP. |
PAS190Mu01 | Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IF. | |
Assay Kits | CLS190Mu01 | CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | CLIA assay for Antigen Detection |
ELS190Mu01 | ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPS190Ra01 | Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAS190Ra01 | Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IP. |
PAS190Ra01 | Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IF. | |
Assay Kits | CLS190Ra01 | CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | CLIA assay for Antigen Detection |
ELS190Ra01 | ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPS190Ca01 | Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAS190Ca01 | Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IP. |
PAS190Ca01 | Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IF. | |
Assay Kits | CLS190Ca01 | CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | CLIA assay for Antigen Detection |
ELS190Ca01 | ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPS190Po01 | Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAS190Po01 | Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IP. |
PAS190Po01 | Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IF. | |
Assay Kits | CLS190Po01 | CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | CLIA assay for Antigen Detection |
ELS190Po01 | ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPS190Bo01 | Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAS190Bo01 | Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IP. |
PAS190Bo01 | Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IF. | |
Assay Kits | CLS190Bo01 | CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | CLIA assay for Antigen Detection |
ELS190Bo01 | ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPS190Rb01 | Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAS190Rb01 | Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IP. |
PAS190Rb01 | Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IF. | |
Assay Kits | CLS190Rb01 | CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | CLIA assay for Antigen Detection |
ELS190Rb01 | ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
---|---|---|---|
Proteins | RPS190Mo01 | Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAS190Mo01 | Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IP. |
PAS190Mo01 | Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) | WB; IHC; ICC; IF. | |
Assay Kits | CLS190Mo01 | CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | CLIA assay for Antigen Detection |
ELS190Mo01 | ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"cDNA cloning of a novel human ubiquitin carrier protein. An antigenic domain specifically recognized by endemic pemphigus foliaceus autoantibodies is encoded in a secondary reading frame of this human epidermal transcript."
J. Biol. Chem. 267:15829-15835(1992) [PubMed] [Europe PMC] [Abstract]
"The DNA sequence and biology of human chromosome 19."
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"E2-EPF UCP targets pVHL for degradation and associates with tumor growth and metastasis."
Nat. Med. 12:809-816(2006) [PubMed] [Europe PMC] [Abstract]
"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
"UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit."
Nat. Cell Biol. 11:1363-1369(2009) [PubMed] [Europe PMC] [Abstract]
"Identification of a physiological E2 module for the human anaphase-promoting complex."
Proc. Natl. Acad. Sci. U.S.A. 106:18213-18218(2009) [PubMed] [Europe PMC] [Abstract]
"The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
"Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne."
Nat. Struct. Mol. Biol. 17:939-947(2010) [PubMed] [Europe PMC] [Abstract]
"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
"Crystal structure of human ubiquitin-conjugating enzyme E2S."
Submitted (FEB-2009) to the PDB data bank