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Ubiquitin Conjugating Enzyme E2S (UBE2S)

E2-EPF; Ubiquitin Carrier Protein; Ubiquitin-Protein Ligase; Ubiquitin-protein ligase S

The ubiquitin-proteasome protein degradation pathway involves 3 enzymes: E1, which forms a thioester bond with ubiquitin; E2, which transiently carries the activated ubiquitin molecule as a thioester; and E3, which transfers the activated ubiquitin from the E2 to the substrate.
UBE2S behaves as an E2 ubiquitin carrier protein.The longest open reading frame (EPF5-ORF2) encodes a deduced 225-amino acid protein with a calculated molecular mass of 24 kD. It shares 38% identity with yeast Ubc4 and is highly similar to ubiquitin carrier proteins in the core region containing the active-site cysteine. Western blot analysis revealed that EPF serum recognized a much shorter protein encoded by an alternate reading frame, EPF5-ORF1.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPS190Hu01 Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAS190Hu01 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IP.
PAS190Hu01 Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IF.
Assay Kits CLS190Hu01 CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) CLIA assay for Antigen Detection
ELS190Hu01 ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPS190Mu01 Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAS190Mu01 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IP.
PAS190Mu01 Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IF.
Assay Kits CLS190Mu01 CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) CLIA assay for Antigen Detection
ELS190Mu01 ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPS190Ra01 Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAS190Ra01 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IP.
PAS190Ra01 Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IF.
Assay Kits CLS190Ra01 CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) CLIA assay for Antigen Detection
ELS190Ra01 ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPS190Ca01 Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAS190Ca01 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IP.
PAS190Ca01 Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IF.
Assay Kits CLS190Ca01 CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) CLIA assay for Antigen Detection
ELS190Ca01 ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPS190Po01 Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAS190Po01 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IP.
PAS190Po01 Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IF.
Assay Kits CLS190Po01 CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) CLIA assay for Antigen Detection
ELS190Po01 ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPS190Bo01 Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAS190Bo01 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IP.
PAS190Bo01 Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IF.
Assay Kits CLS190Bo01 CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) CLIA assay for Antigen Detection
ELS190Bo01 ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPS190Rb01 Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAS190Rb01 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IP.
PAS190Rb01 Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IF.
Assay Kits CLS190Rb01 CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) CLIA assay for Antigen Detection
ELS190Rb01 ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPS190Mo01 Recombinant Ubiquitin Conjugating Enzyme E2S (UBE2S) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAS190Mo01 Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IP.
PAS190Mo01 Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2S (UBE2S) WB; IHC; ICC; IF.
Assay Kits CLS190Mo01 CLIA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) CLIA assay for Antigen Detection
ELS190Mo01 ELISA Kit for Ubiquitin Conjugating Enzyme E2S (UBE2S) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"cDNA cloning of a novel human ubiquitin carrier protein. An antigenic domain specifically recognized by endemic pemphigus foliaceus autoantibodies is encoded in a secondary reading frame of this human epidermal transcript."
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"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"E2-EPF UCP targets pVHL for degradation and associates with tumor growth and metastasis."
Nat. Med. 12:809-816(2006) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit."
Nat. Cell Biol. 11:1363-1369(2009) [PubMed] [Europe PMC] [Abstract]

"Identification of a physiological E2 module for the human anaphase-promoting complex."
Proc. Natl. Acad. Sci. U.S.A. 106:18213-18218(2009) [PubMed] [Europe PMC] [Abstract]

"The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]

"Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne."
Nat. Struct. Mol. Biol. 17:939-947(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human ubiquitin-conjugating enzyme E2S."
Submitted (FEB-2009) to the PDB data bank