Sphingosine Kinase 1 (SPHK1)

SPN	S103
HGNC	11240
MGI	1316649
PubMed	16980623
RGD	620048
UniProt	Q9NYA1
Wiki	SPHK1

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1. Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor and serum.Sequence comparisons of the mammalian SPHK proteins and SPHKs from other organisms identified 5 regions that are highly conserved. Northern blot analysis of mouse adult tissues showed ubiquitous expression of Sphk1, with the highest levels found in lung and spleen.

Reference

"Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution."
Gene 251:19-26(2000) [PubMed] [Europe PMC] [Abstract]

"Functional characterization of human sphingosine kinase-1."
FEBS Lett. 473:81-84(2000) [PubMed] [Europe PMC] [Abstract]

"Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes."
Biochem. J. 350:429-441(2000) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Cloning and characterization of a protein kinase A anchoring protein (AKAP)-related protein that interacts with and regulates sphingosine kinase 1 activity."
J. Biol. Chem. 277:32947-32953(2002) [PubMed] [Europe PMC] [Abstract]

"Identification of functional nuclear export sequences in human sphingosine kinase 1."
Biochem. Biophys. Res. Commun. 311:168-173(2003) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Translocation of sphingosine kinase 1 to the plasma membrane is mediated by calcium- and integrin-binding protein 1."
J. Biol. Chem. 285:483-492(2010) [PubMed] [Europe PMC] [Abstract]

"Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2."
Nature 465:1084-1088(2010) [PubMed] [Europe PMC] [Abstract]

"Molecular basis of sphingosine kinase 1 substrate recognition and catalysis."
Structure 21:798-809(2013) [PubMed] [Europe PMC] [Abstract]