Replication Factor C2 (RFC2)

A1; RFC40; Activator 1; Activator 1 40 kDa subunit; Activator 1 subunit 2; Replication factor C 40 kDa subunit

SPN	R722
HGNC	9970
MGI	1341868
PubMed	18245774
RGD	621198
UniProt	P35250
Wiki	RFC2

Studies with the complete 5-subunit holoenzyme indicated that the large subunit binds to DNA and the 40-kD subunit binds ATP. The other subunits may play discrete roles in the elongation process catalyzed by polymerase.

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. Replication Factor C2 isthe 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPR722Hu01	Recombinant Replication Factor C2 (RFC2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAR722Hu01	Monoclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IP.
Antibodies	PAR722Hu01	Polyclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IF.
Assay Kits	CLR722Hu01	CLIA Kit for Replication Factor C2 (RFC2)	CLIA assay for Antigen Detection
Assay Kits	ELR722Hu01	ELISA Kit for Replication Factor C2 (RFC2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPR722Mu01	Recombinant Replication Factor C2 (RFC2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAR722Mu01	Monoclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IP.
Antibodies	PAR722Mu01	Polyclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IF.
Assay Kits	CLR722Mu01	CLIA Kit for Replication Factor C2 (RFC2)	CLIA assay for Antigen Detection
Assay Kits	ELR722Mu01	ELISA Kit for Replication Factor C2 (RFC2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPR722Ra01	Recombinant Replication Factor C2 (RFC2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAR722Ra01	Monoclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IP.
Antibodies	PAR722Ra01	Polyclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IF.
Assay Kits	CLR722Ra01	CLIA Kit for Replication Factor C2 (RFC2)	CLIA assay for Antigen Detection
Assay Kits	ELR722Ra01	ELISA Kit for Replication Factor C2 (RFC2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPR722Ca01	Recombinant Replication Factor C2 (RFC2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAR722Ca01	Monoclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IP.
Antibodies	PAR722Ca01	Polyclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IF.
Assay Kits	CLR722Ca01	CLIA Kit for Replication Factor C2 (RFC2)	CLIA assay for Antigen Detection
Assay Kits	ELR722Ca01	ELISA Kit for Replication Factor C2 (RFC2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPR722Po01	Recombinant Replication Factor C2 (RFC2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAR722Po01	Monoclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IP.
Antibodies	PAR722Po01	Polyclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IF.
Assay Kits	CLR722Po01	CLIA Kit for Replication Factor C2 (RFC2)	CLIA assay for Antigen Detection
Assay Kits	ELR722Po01	ELISA Kit for Replication Factor C2 (RFC2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPR722Bo01	Recombinant Replication Factor C2 (RFC2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAR722Bo01	Monoclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IP.
Antibodies	PAR722Bo01	Polyclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IF.
Assay Kits	CLR722Bo01	CLIA Kit for Replication Factor C2 (RFC2)	CLIA assay for Antigen Detection
Assay Kits	ELR722Bo01	ELISA Kit for Replication Factor C2 (RFC2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPR722Rb01	Recombinant Replication Factor C2 (RFC2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAR722Rb01	Monoclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IP.
Antibodies	PAR722Rb01	Polyclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IF.
Assay Kits	CLR722Rb01	CLIA Kit for Replication Factor C2 (RFC2)	CLIA assay for Antigen Detection
Assay Kits	ELR722Rb01	ELISA Kit for Replication Factor C2 (RFC2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPR722Mo01	Recombinant Replication Factor C2 (RFC2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAR722Mo01	Monoclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IP.
Antibodies	PAR722Mo01	Polyclonal Antibody to Replication Factor C2 (RFC2)	WB; IHC; ICC; IF.
Assay Kits	CLR722Mo01	CLIA Kit for Replication Factor C2 (RFC2)	CLIA assay for Antigen Detection
Assay Kits	ELR722Mo01	ELISA Kit for Replication Factor C2 (RFC2)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Sequence and expression in Escherichia coli of the 40-kDa subunit of activator 1 (replication factor C) of HeLa cells."
Proc. Natl. Acad. Sci. U.S.A. 89:2516-2520(1992) [PubMed] [Europe PMC] [Abstract]

"Comparative genomic sequence analysis of the Williams syndrome region (LIMK1-RFC2) of human chromosome 7q11.23."
Mamm. Genome 11:890-898(2000) [PubMed] [Europe PMC] [Abstract]

"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence of human chromosome 7."
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The second subunit of the replication factor C complex (RFC40) and the regulatory subunit (RIalpha) of protein kinase A form a protein complex promoting cell survival."
Cell Cycle 4:323-329(2005) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]