Phosphodiesterase 5A, cGMP Specific (PDE5A)

CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase

SPN	Q093
HGNC	8784
MGI	2651499
PubMed	21215707
RGD	620995
UniProt	O76074
Wiki	PDE5A

PDE5A encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

The predicted 875-amino acid human protein is approximately 96% identical to bovine PDE5A. Like the bovine protein, human PDE5A contains a cGMP-binding domain in its N-terminal portion and a catalytic domain in its C-terminal region. Recombinant PDE5A protein hydrolyzed cGMP in vitro.

Reference

"Isolation and characterization of cDNAs encoding PDE5A, a human cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase."
Gene 216:139-147(1998) [PubMed] [Europe PMC] [Abstract]

"Expression, structure and chromosomal localization of the human cGMP-binding cGMP-specific phosphodiesterase PDE5A gene."
Eur. J. Biochem. 255:391-399(1998) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning and expression of human cGMP-binding cGMP-specific phosphodiesterase."
Biochem. Biophys. Res. Commun. 247:249-254(1998) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Multiple cyclic nucleotide phosphodiesterases in human trabecular meshwork cells."
Invest. Ophthalmol. Vis. Sci. 40:1745-1752(1999) [PubMed] [Europe PMC] [Abstract]

"Regulation of cGMP-specific phosphodiesterase (PDE5) phosphorylation in smooth muscle cells."
J. Biol. Chem. 277:3310-3317(2002) [PubMed] [Europe PMC] [Abstract]

"Structure of the catalytic domain of human phosphodiesterase 5 with bound drug molecules."
Nature 425:98-102(2003) [PubMed] [Europe PMC] [Abstract]

"Phosphodiesterase 9A controls nitric-oxide-independent cGMP and hypertrophic heart disease."
Nature 519:472-476(2015) [PubMed] [Europe PMC] [Abstract]