Glycine-N-Methyltransferase (GNMT)

SPN	P928
HGNC	4415
MGI	1202304
PubMed	22687285
RGD	2719
UniProt	Q14749
Wiki	GNMT

Glycine N-methyltransferase (GNMT; EC 2.1.1.20) catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor.GNMT acts as an enzyme to regulate the ratio of S-adenosylmethionine to S-adenosylhomocysteine (AdoHcy) and participates in the detoxification pathway in liver cells.

These unbiased metabolomic profiles were able to distinguish benign prostate, clinically localized prostate cancer, and metastatic disease. Sarcosine, an N-methyl derivative of the amino acid glycine, was identified as a differential metabolite that is highly increased during prostate cancer progression to metastasis and can be detected noninvasively in urine. Sarcosine levels were also increased in invasive prostate cancer cell lines relative to benign prostate epithelial cells.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP928Hu01	Recombinant Glycine-N-Methyltransferase (GNMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP928Hu01	Monoclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IP.
Antibodies	PAP928Hu01	Polyclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IF.
Assay Kits	CLP928Hu01	CLIA Kit for Glycine-N-Methyltransferase (GNMT)	CLIA assay for Antigen Detection
Assay Kits	ELP928Hu01	ELISA Kit for Glycine-N-Methyltransferase (GNMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP928Mu01	Recombinant Glycine-N-Methyltransferase (GNMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP928Mu01	Monoclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IP.
Antibodies	PAP928Mu01	Polyclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IF.
Assay Kits	CLP928Mu01	CLIA Kit for Glycine-N-Methyltransferase (GNMT)	CLIA assay for Antigen Detection
Assay Kits	ELP928Mu01	ELISA Kit for Glycine-N-Methyltransferase (GNMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP928Ra01	Recombinant Glycine-N-Methyltransferase (GNMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP928Ra01	Monoclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IP.
Antibodies	PAP928Ra01	Polyclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IF.
Assay Kits	CLP928Ra01	CLIA Kit for Glycine-N-Methyltransferase (GNMT)	CLIA assay for Antigen Detection
Assay Kits	ELP928Ra01	ELISA Kit for Glycine-N-Methyltransferase (GNMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP928Ca01	Recombinant Glycine-N-Methyltransferase (GNMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP928Ca01	Monoclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IP.
Antibodies	PAP928Ca01	Polyclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IF.
Assay Kits	CLP928Ca01	CLIA Kit for Glycine-N-Methyltransferase (GNMT)	CLIA assay for Antigen Detection
Assay Kits	ELP928Ca01	ELISA Kit for Glycine-N-Methyltransferase (GNMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP928Po01	Recombinant Glycine-N-Methyltransferase (GNMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP928Po01	Monoclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IP.
Antibodies	PAP928Po01	Polyclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IF.
Assay Kits	CLP928Po01	CLIA Kit for Glycine-N-Methyltransferase (GNMT)	CLIA assay for Antigen Detection
Assay Kits	ELP928Po01	ELISA Kit for Glycine-N-Methyltransferase (GNMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP928Bo01	Recombinant Glycine-N-Methyltransferase (GNMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP928Bo01	Monoclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IP.
Antibodies	PAP928Bo01	Polyclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IF.
Assay Kits	CLP928Bo01	CLIA Kit for Glycine-N-Methyltransferase (GNMT)	CLIA assay for Antigen Detection
Assay Kits	ELP928Bo01	ELISA Kit for Glycine-N-Methyltransferase (GNMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP928Rb01	Recombinant Glycine-N-Methyltransferase (GNMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP928Rb01	Monoclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IP.
Antibodies	PAP928Rb01	Polyclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IF.
Assay Kits	CLP928Rb01	CLIA Kit for Glycine-N-Methyltransferase (GNMT)	CLIA assay for Antigen Detection
Assay Kits	ELP928Rb01	ELISA Kit for Glycine-N-Methyltransferase (GNMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP928Mo01	Recombinant Glycine-N-Methyltransferase (GNMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP928Mo01	Monoclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IP.
Antibodies	PAP928Mo01	Polyclonal Antibody to Glycine-N-Methyltransferase (GNMT)	WB; IHC; ICC; IF.
Assay Kits	CLP928Mo01	CLIA Kit for Glycine-N-Methyltransferase (GNMT)	CLIA assay for Antigen Detection
Assay Kits	ELP928Mo01	ELISA Kit for Glycine-N-Methyltransferase (GNMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Characterization of glycine-N-methyltransferase-gene expression in human hepatocellular carcinoma."
Int. J. Cancer 75:787-793(1998) [PubMed] [Europe PMC] [Abstract]

"Genomic structure, expression, and chromosomal localization of the human glycine N-methyltransferase gene."
Genomics 66:43-47(2000) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and analysis of human chromosome 6."
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers."
Comp. Biochem. Physiol. 106B:601-611(1993) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes."
Proteins 57:331-337(2004) [PubMed] [Europe PMC] [Abstract]

"Destabilization of human glycine N-methyltransferase by H176N mutation."
Protein Sci. 16:1957-1964(2007) [PubMed] [Europe PMC] [Abstract]

"Mutations in human glycine N-methyltransferase give insights into its role in methionine metabolism."
Hum. Genet. 110:68-74(2002) [PubMed] [Europe PMC] [Abstract]

"Effect of naturally occurring mutations in human glycine N-methyltransferase on activity and conformation."
Biochem. Biophys. Res. Commun. 312:1067-1072(2003) [PubMed] [Europe PMC] [Abstract]

"Glycine N-methyltransferase deficiency: a new patient with a novel mutation."
J. Inherit. Metab. Dis. 26:745-759(2003) [PubMed] [Europe PMC] [Abstract]