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Thymidylate Synthetase (TYMS)

TS; TMS; TSase; Thymidylate Synthase

Thymidylate synthetase (EC 2.1.1.45) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product.
As an anti-cancer chemotherapy target, thymidylate synthetase can be inhibited by the thymidylate synthase inhibitors such as fluorinated pyrimidine fluorouracil, or certain folate analogues, including most notably raltitrexed (trade name Tomudex).

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPP787Hu01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAP787Hu01 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
PAP787Hu01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IF.
Assay Kits CLP787Hu01 CLIA Kit for Thymidylate Synthetase (TYMS) CLIA assay for Antigen Detection
ELP787Hu01 ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPP787Mu01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAP787Mu01 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
PAP787Mu01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IF.
Assay Kits CLP787Mu01 CLIA Kit for Thymidylate Synthetase (TYMS) CLIA assay for Antigen Detection
ELP787Mu01 ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPP787Ra01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAP787Ra01 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
PAP787Ra01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IF.
Assay Kits CLP787Ra01 CLIA Kit for Thymidylate Synthetase (TYMS) CLIA assay for Antigen Detection
ELP787Ra01 ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPP787Ca01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAP787Ca01 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
PAP787Ca01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IF.
Assay Kits CLP787Ca01 CLIA Kit for Thymidylate Synthetase (TYMS) CLIA assay for Antigen Detection
ELP787Ca01 ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPP787Po01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAP787Po01 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
PAP787Po01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IF.
Assay Kits CLP787Po01 CLIA Kit for Thymidylate Synthetase (TYMS) CLIA assay for Antigen Detection
ELP787Po01 ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPP787Bo01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAP787Bo01 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
PAP787Bo01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IF.
Assay Kits CLP787Bo01 CLIA Kit for Thymidylate Synthetase (TYMS) CLIA assay for Antigen Detection
ELP787Bo01 ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPP787Rb01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAP787Rb01 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
PAP787Rb01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IF.
Assay Kits CLP787Rb01 CLIA Kit for Thymidylate Synthetase (TYMS) CLIA assay for Antigen Detection
ELP787Rb01 ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPP787Mo01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAP787Mo01 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
PAP787Mo01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IF.
Assay Kits CLP787Mo01 CLIA Kit for Thymidylate Synthetase (TYMS) CLIA assay for Antigen Detection
ELP787Mo01 ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Nucleotide sequence of a functional cDNA for human thymidylate synthase."
Nucleic Acids Res. 13:2035-2043(1985) [PubMed] [Europe PMC] [Abstract]

"Structural and functional analysis of the human thymidylate synthase gene."
J. Biol. Chem. 265:20277-20284(1990) [PubMed] [Europe PMC] [Abstract]

"Differential alternative splicing expressions of thymidylate synthase isoforms."
Cancer Lett. 193:127-131(2003) [PubMed] [Europe PMC] [Abstract]

"DNA sequence and analysis of human chromosome 18."
Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Human thymidylate synthase gene: isolation of phage clones which cover a functionally active gene and structural analysis of the region upstream from the translation initiation codon."
J. Biochem. 106:575-583(1989) [PubMed] [Europe PMC] [Abstract]

"Purification and NH2-terminal amino acid sequence of human thymidylate synthase in an overproducing transformant of mouse FM3A cells."
J. Biochem. 97:845-850(1985) [PubMed] [Europe PMC] [Abstract]

"Expression of human thymidylate synthase in Escherichia coli."
J. Biol. Chem. 264:9145-9148(1989) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria."
Proc. Natl. Acad. Sci. U.S.A. 108:15163-15168(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site."
Biochemistry 34:16279-16287(1995) [PubMed] [Europe PMC] [Abstract]

"Human thymidylate synthase is in the closed conformation when complexed with dUMP and raltitrexed, an antifolate drug."
Biochemistry 40:1897-1902(2001) [PubMed] [Europe PMC] [Abstract]

"Structure of human thymidylate synthase suggests advantages of chemotherapy with noncompetitive inhibitors."
J. Biol. Chem. 276:14170-14177(2001) [PubMed] [Europe PMC] [Abstract]