Pleckstrin Homology Domain Containing Family A, Member 1 (PLEKHA1)

TAPP1; Tandem PH Domain Containing Protein-1; PH domain-containing family A member 1

SPN	O877
HGNC	14335
MGI	2442213
PubMed	17000705
RGD	1564153
UniProt	Q9HB21
Wiki	PLEKHA1

The last 3 C-terminal amino acids of TAPP1 are predicted to interact with PDZ domain-containing proteins. TAPP1 and TAPP2 share 58% identity over the first 300 amino acids, which encompass both PH domains. There is little homology in the C termini. Northern blot analysis detected a 4.0-kb transcript expressed in all tissues examined, with highest levels in skeletal muscle, spleen, lung, thymus, and placenta.

TAPP1 possessed a 5-fold higher affinity for PtdIns(3,4)P2 than TAPP2. Mutation of the conserved arg212 to leucine within the PPBM of the second PH domain of TAPP1 reduced or eliminated PtdIns(3,4)P2 binding. The N-terminal PH domain contributed to the affinity with which the C-terminal PH domain bound to PtdIns(3,4)P2.

Reference

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Biochem. J. 351:19-31(2000) [PubMed] [Europe PMC] [Abstract]

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Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo."
Biochem. J. 361:525-536(2002) [PubMed] [Europe PMC] [Abstract]

"TAPP1 and TAPP2 are targets of phosphatidylinositol 3-kinase signaling in B cells: sustained plasma membrane recruitment triggered by the B-cell antigen receptor."
Mol. Cell. Biol. 22:5479-5491(2002) [PubMed] [Europe PMC] [Abstract]

"Interaction of the protein tyrosine phosphatase PTPL1 with the PtdIns(3,4)P2-binding adaptor protein TAPP1."
Biochem. J. 376:525-535(2003) [PubMed] [Europe PMC] [Abstract]

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Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the phosphatidylinositol 3,4-bisphosphate-binding pleckstrin homology (PH) domain of tandem PH-domain-containing protein 1 (TAPP1): molecular basis of lipid specificity."
Biochem. J. 358:287-294(2001) [PubMed] [Europe PMC] [Abstract]