Hepatocyte Growth Factor Activator (HGFAC)

HGFA; HGFAP; HGF Activator

SPN	O837
HGNC	4894
MGI	1859281
PubMed	22877752
RGD	70909
UniProt	Q04756
Wiki	HGFAC

HGFAC belongs to peptidase family S1. It is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Miyazawa et al. (1993) determined the partial amino acid sequence of the homologous human serine protease and used it to design degenerate primers to screen a human liver cDNA library. They identified a 655-amino acid inactive precursor protein, designated hepatocyte growth factor activator (HGFAC), with a calculated molecular mass of 70,681 Da. They determined that the active protein purified from serum is derived from the C terminus of the precursor by proteolytic cleavage of the bonds between arg372-val373 and arg407-ile408.

Reference

"Molecular cloning and sequence analysis of the cDNA for a human serine protease responsible for activation of hepatocyte growth factor. Structural similarity of the protease precursor to blood coagulation factor XII."
J. Biol. Chem. 268:10024-10028(1993) [PubMed] [Europe PMC] [Abstract]

"Structural organization and chromosomal localization of the human hepatocyte growth factor activator gene -- phylogenetic and functional relationship with blood coagulation factor XII, urokinase, and tissue-type plasminogen activator."
Eur. J. Biochem. 258:355-361(1998) [PubMed] [Europe PMC] [Abstract]

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

"Conformational lability in serine protease active sites: structures of hepatocyte growth factor activator (HGFA) alone and with the inhibitory domain from HGFA inhibitor-1B."
J. Mol. Biol. 346:1335-1349(2005) [PubMed] [Europe PMC] [Abstract]

"Structural insight into distinct mechanisms of protease inhibition by antibodies."
Proc. Natl. Acad. Sci. U.S.A. 104:19784-19789(2007) [PubMed] [Europe PMC] [Abstract]