Glucuronic Acid Epimerase (GLCE)

HSEPI; D-Glucuronyl C5-Epimerase; UDP-Glucuronic Acid Epimerase; Heparan Sulfate Epimerase; Heparin/heparan sulfate:glucuronic acid C5-epimerase

SPN	O725
HGNC	17855
MGI	2136405
PubMed	21488854
RGD	1565253
UniProt	O94923
Wiki	GLCE

Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines.

The transcript contains a repetitive element in its 3-prime UTR. The deduced 609-amino acid protein shares 98.9% identity with bovine C5-glucuronyl epimerase over 444 amino acids. RT-PCR ELISA detected ubiquitous GLCE expression, with highest levels in ovary and brain. Within brain, expression was highest in amygdala, cerebellum, and subthalamic nucleus.

Reference

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"Using engineered 2-O-sulfotransferase to determine the activity of heparan sulfate C5-epimerase and its mutants."
J. Biol. Chem. 285:11106-11113(2010) [PubMed] [Europe PMC] [Abstract]

"Uncovering a biphasic catalytic mode of C5-epimerase in heparan sulfate biosynthesis."
J. Biol. Chem. 287:20996-21002(2012) [PubMed] [Europe PMC] [Abstract]