Cleavage And Polyadenylation Specific Factor 1 (CPSF1)
CPSF160; Cleavage and polyadenylation specificity factor 160 kDa subunit
Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex.
The 1,442-amino acid protein contains a bipartite nuclear localization signal and an RNA-binding domain. Northern blot analysis revealed expression of a 5.0-kb transcript. Immunofluorescence microscopy and Western blot analysis showed expression of an exclusively nuclear 160-kD protein.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPO399Hu01 | Recombinant Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAO399Hu01 | Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IP. |
| PAO399Hu01 | Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLO399Hu01 | CLIA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | CLIA assay for Antigen Detection |
| ELO399Hu01 | ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPO399Mu01 | Recombinant Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAO399Mu01 | Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IP. |
| PAO399Mu01 | Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLO399Mu01 | CLIA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | CLIA assay for Antigen Detection |
| ELO399Mu01 | ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPO399Ra01 | Recombinant Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAO399Ra01 | Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IP. |
| PAO399Ra01 | Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLO399Ra01 | CLIA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | CLIA assay for Antigen Detection |
| ELO399Ra01 | ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPO399Ca01 | Recombinant Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAO399Ca01 | Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IP. |
| PAO399Ca01 | Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLO399Ca01 | CLIA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | CLIA assay for Antigen Detection |
| ELO399Ca01 | ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPO399Po01 | Recombinant Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAO399Po01 | Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IP. |
| PAO399Po01 | Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLO399Po01 | CLIA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | CLIA assay for Antigen Detection |
| ELO399Po01 | ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPO399Bo01 | Recombinant Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAO399Bo01 | Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IP. |
| PAO399Bo01 | Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLO399Bo01 | CLIA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | CLIA assay for Antigen Detection |
| ELO399Bo01 | ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPO399Rb01 | Recombinant Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAO399Rb01 | Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IP. |
| PAO399Rb01 | Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLO399Rb01 | CLIA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | CLIA assay for Antigen Detection |
| ELO399Rb01 | ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPO399Mo01 | Recombinant Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAO399Mo01 | Monoclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IP. |
| PAO399Mo01 | Polyclonal Antibody to Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLO399Mo01 | CLIA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | CLIA assay for Antigen Detection |
| ELO399Mo01 | ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation."
Genes Dev. 9:2672-2683(1995) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"SRm160 splicing coactivator promotes transcript 3'-end cleavage."
Mol. Cell. Biol. 22:148-160(2002) [PubMed] [Europe PMC] [Abstract]
"Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase."
EMBO J. 23:616-626(2004) [PubMed] [Europe PMC] [Abstract]
"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
"The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA."
EMBO J. 29:4132-4145(2010) [PubMed] [Europe PMC] [Abstract]
"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]