Arylformamidase (AFMID) is the second enzyme of the kynurenine pathway metabolizing tryptophan to nicotinic acid and nicotinamide adenine dinucleotide cofactors. Inhibition of AFMID by organophosphorus insecticides in developing chicken embryos is correlated with lowered NAD levels and severe teratogenesis.
The cDNA sequence previously identified for mouse liver AFMID was cloned and expressed in Escherichia coli. Residues identified as potential catalytic triad members through sequence motif and homology modeling were mutated to alanine to probe their contributions to enzyme activity. The wild-type and mutant AFMIDs were expressed as amino terminal 6 x His-tagged recombinant proteins to facilitate purification. Three chromatography steps isolated highly purified proteins for enzyme activity comparisons.
Organism species: Homo sapiens (Human)
PRODUCT TYPE
CATALOG NO.
PRODUCT NAME
APPLICATIONS
Proteins
RPO099Hu01
Recombinant Arylformamidase (AFMID)
Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies
MAO099Hu01
Monoclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IP.
PAO099Hu01
Polyclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IF.
Assay Kits
CLO099Hu01
CLIA Kit for Arylformamidase (AFMID)
CLIA assay for Antigen Detection
ELO099Hu01
ELISA Kit for Arylformamidase (AFMID)
Enzyme-linked immunosorbent assay for Antigen Detection
Organism species: Mus musculus (Mouse)
PRODUCT TYPE
CATALOG NO.
PRODUCT NAME
APPLICATIONS
Proteins
RPO099Mu01
Recombinant Arylformamidase (AFMID)
Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies
MAO099Mu01
Monoclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IP.
PAO099Mu01
Polyclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IF.
Assay Kits
CLO099Mu01
CLIA Kit for Arylformamidase (AFMID)
CLIA assay for Antigen Detection
ELO099Mu01
ELISA Kit for Arylformamidase (AFMID)
Enzyme-linked immunosorbent assay for Antigen Detection
Organism species: Rattus norvegicus (Rat)
PRODUCT TYPE
CATALOG NO.
PRODUCT NAME
APPLICATIONS
Proteins
RPO099Ra01
Recombinant Arylformamidase (AFMID)
Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies
MAO099Ra01
Monoclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IP.
PAO099Ra01
Polyclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IF.
Assay Kits
CLO099Ra01
CLIA Kit for Arylformamidase (AFMID)
CLIA assay for Antigen Detection
ELO099Ra01
ELISA Kit for Arylformamidase (AFMID)
Enzyme-linked immunosorbent assay for Antigen Detection
Organism species: Canis familiaris; Canine (Dog)
PRODUCT TYPE
CATALOG NO.
PRODUCT NAME
APPLICATIONS
Proteins
RPO099Ca01
Recombinant Arylformamidase (AFMID)
Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies
MAO099Ca01
Monoclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IP.
PAO099Ca01
Polyclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IF.
Assay Kits
CLO099Ca01
CLIA Kit for Arylformamidase (AFMID)
CLIA assay for Antigen Detection
ELO099Ca01
ELISA Kit for Arylformamidase (AFMID)
Enzyme-linked immunosorbent assay for Antigen Detection
Organism species: Sus scrofa; Porcine (Pig)
PRODUCT TYPE
CATALOG NO.
PRODUCT NAME
APPLICATIONS
Proteins
RPO099Po01
Recombinant Arylformamidase (AFMID)
Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies
MAO099Po01
Monoclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IP.
PAO099Po01
Polyclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IF.
Assay Kits
CLO099Po01
CLIA Kit for Arylformamidase (AFMID)
CLIA assay for Antigen Detection
ELO099Po01
ELISA Kit for Arylformamidase (AFMID)
Enzyme-linked immunosorbent assay for Antigen Detection
Organism species: Bos taurus; Bovine (Cattle)
PRODUCT TYPE
CATALOG NO.
PRODUCT NAME
APPLICATIONS
Proteins
RPO099Bo01
Recombinant Arylformamidase (AFMID)
Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies
MAO099Bo01
Monoclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IP.
PAO099Bo01
Polyclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IF.
Assay Kits
CLO099Bo01
CLIA Kit for Arylformamidase (AFMID)
CLIA assay for Antigen Detection
ELO099Bo01
ELISA Kit for Arylformamidase (AFMID)
Enzyme-linked immunosorbent assay for Antigen Detection
Organism species: Oryctolagus cuniculus (Rabbit)
PRODUCT TYPE
CATALOG NO.
PRODUCT NAME
APPLICATIONS
Proteins
RPO099Rb01
Recombinant Arylformamidase (AFMID)
Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies
MAO099Rb01
Monoclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IP.
PAO099Rb01
Polyclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IF.
Assay Kits
CLO099Rb01
CLIA Kit for Arylformamidase (AFMID)
CLIA assay for Antigen Detection
ELO099Rb01
ELISA Kit for Arylformamidase (AFMID)
Enzyme-linked immunosorbent assay for Antigen Detection
Organism species: Rhesus monkey (Simian)
PRODUCT TYPE
CATALOG NO.
PRODUCT NAME
APPLICATIONS
Proteins
RPO099Mo01
Recombinant Arylformamidase (AFMID)
Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies
MAO099Mo01
Monoclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IP.
PAO099Mo01
Polyclonal Antibody to Arylformamidase (AFMID)
WB; IHC; ICC; IF.
Assay Kits
CLO099Mo01
CLIA Kit for Arylformamidase (AFMID)
CLIA assay for Antigen Detection
ELO099Mo01
ELISA Kit for Arylformamidase (AFMID)
Enzyme-linked immunosorbent assay for Antigen Detection
