Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2)
SPRED2 is a member of the Sprouty/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade.
deduced 411-amino acid protein contains an N-terminal Enabled (ENA)/VASP homology-1 (EVH1) domain, a central KIT-binding domain (KBD), and a C-terminal SPRY domain. Endogenous Spred2 localized to the membrane in rat PC12 cells, and mutation analysis determined that the C-terminal SPRY domain was required for plasma membrane localization.Inhibition appeared to occur through the formation of a Spred-Ras complex that inhibited activation of MAP kinase by suppressing phosphorylation and activation of Raf.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPN371Hu01 | Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAN371Hu01 | Monoclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IP. |
| PAN371Hu01 | Polyclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLN371Hu01 | CLIA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | CLIA assay for Antigen Detection |
| ELN371Hu01 | ELISA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPN371Mu01 | Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAN371Mu01 | Monoclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IP. |
| PAN371Mu01 | Polyclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLN371Mu01 | CLIA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | CLIA assay for Antigen Detection |
| ELN371Mu01 | ELISA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPN371Ra01 | Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAN371Ra01 | Monoclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IP. |
| PAN371Ra01 | Polyclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLN371Ra01 | CLIA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | CLIA assay for Antigen Detection |
| ELN371Ra01 | ELISA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPN371Ca01 | Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAN371Ca01 | Monoclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IP. |
| PAN371Ca01 | Polyclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLN371Ca01 | CLIA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | CLIA assay for Antigen Detection |
| ELN371Ca01 | ELISA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPN371Po01 | Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAN371Po01 | Monoclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IP. |
| PAN371Po01 | Polyclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLN371Po01 | CLIA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | CLIA assay for Antigen Detection |
| ELN371Po01 | ELISA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPN371Bo01 | Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAN371Bo01 | Monoclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IP. |
| PAN371Bo01 | Polyclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLN371Bo01 | CLIA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | CLIA assay for Antigen Detection |
| ELN371Bo01 | ELISA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPN371Rb01 | Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAN371Rb01 | Monoclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IP. |
| PAN371Rb01 | Polyclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLN371Rb01 | CLIA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | CLIA assay for Antigen Detection |
| ELN371Rb01 | ELISA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPN371Mo01 | Recombinant Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAN371Mo01 | Monoclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IP. |
| PAN371Mo01 | Polyclonal Antibody to Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLN371Mo01 | CLIA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | CLIA assay for Antigen Detection |
| ELN371Mo01 | ELISA Kit for Sprouty Related, EVH1 Domain Containing Protein 2 (SPRED2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Distinct requirements for the Sprouty domain for functional activity of Spred proteins."
Biochem. J. 388:445-454(2005) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Expression and subcellular localization of Spred proteins in mouse and human tissues."
Histochem. Cell Biol. 122:527-538(2004) [PubMed] [Europe PMC] [Abstract]
"Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination."
Biochem. Biophys. Res. Commun. 351:1018-1023(2006) [PubMed] [Europe PMC] [Abstract]
"1H, 13C and 15N resonance assignment of the human Spred2 EVH1 domain."
J. Biomol. NMR 29:435-436(2004) [PubMed] [Europe PMC] [Abstract]