Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4)

RBAF600; p600; ZUBR1; Zinc Finger,UBR1 Type 1; N-recognin-4; 600 kDa retinoblastoma protein-associated factor; Retinoblastoma-associated factor of 600 kDa

SPN	N316
HGNC	30313
MGI	1916366
PubMed	23555265
RGD	1563121
UniProt	Q5T4S7
Wiki	UBR4

RBAF600 associates with RB in the nucleus and with Ca(2+)-bound calmodulin in the cytoplasm.p600 and RB appears to act as a chromatin scaffold, and in the cytoplasm p600 and clathrin form a meshwork structure which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interfering RNA abrogated integrin-mediated ruffled membrane formation and prevented activation of integrin-mediated survival pathways. The deduced 5,183-amino acid protein has a calculated molecular mass of 573.5 kD. Immunostaining of human foreskin fibroblasts localized p600 in a continuous meshwork pattern from the nucleus to the cytoplasm, passing through the nuclear envelope. In the cytoplasm, p600 was concentrated at the leading edge of membrane structures.

Reference

"p600, a unique protein required for membrane morphogenesis and cell survival."
Proc. Natl. Acad. Sci. U.S.A. 102:15093-15098(2005) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

, Submitted (JUL-2007) to UniProtKB

"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]

"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain."
DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The response of autologous T cells to a human melanoma is dominated by mutated neoantigens."
Proc. Natl. Acad. Sci. U.S.A. 102:16013-16018(2005) [PubMed] [Europe PMC] [Abstract]

"The full-ORF clone resource of the German cDNA consortium."
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

"Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600."
Proc. Natl. Acad. Sci. U.S.A. 102:11492-11497(2005) [PubMed] [Europe PMC] [Abstract]

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth."
Mol. Cell 51:506-518(2013) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"The consensus coding sequences of human breast and colorectal cancers."
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]