Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1)

ZA2G; ZAG

SPN	N158
HGNC	910
MGI	103163
PubMed	17724461
RGD	2187
UniProt	P25311
Wiki	AZGP1

AZGP1 expresses a soluble protein that stimulates lipolysis, induces a reduction in body fat in mice, is associated with the cachexia related to cancer, and is known to be expressed in secretory cells of lung epithelium. In 2009, it was found that smoking increases expression of this gene, which is why smoking cessation leads to weight gain.

AZGP1 gene spans over 9.7 kb and that its overall organization and nucleotide sequence resemble those of the first 4 exons of class I MHC genes. However, it differs from those genes by its lack of coding information for transmembrane and cytoplasmic domains typical of MHC genes, which is consistent with its presence as a soluble protein in various physiologic and pathologic fluids. The human genome also contains a putative AZGP1 pseudogene.

Reference

"Human Zn-alpha 2-glycoprotein cDNA cloning and expression analysis in benign and malignant breast tissues."
FEBS Lett. 290:247-249(1991) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning and chromosomal assignment of the gene for human Zn-alpha 2-glycoprotein."
Biochemistry 32:12968-12976(1993) [PubMed] [Europe PMC] [Abstract]

"Human Zn-alpha 2-glycoprotein: complete genomic sequence, identification of a related pseudogene and relationship to class I major histocompatibility complex genes."
Genomics 18:575-587(1993) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence of human chromosome 7."
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]

"Human chromosome 7: DNA sequence and biology."
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Cloning and nucleotide sequence of a human Zn-alpha 2-glycoprotein cDNA and chromosomal assignment of its gene."
Biochem. Biophys. Res. Commun. 177:696-703(1991) [PubMed] [Europe PMC] [Abstract]

"Complete amino acid sequence of human plasma Zn-alpha 2-glycoprotein and its homology to histocompatibility antigens."
Proc. Natl. Acad. Sci. U.S.A. 85:679-683(1988) [PubMed] [Europe PMC] [Abstract]

"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]

"Biochemical characterization and crystallization of human Zn-alpha2-glycoprotein, a soluble class I major histocompatibility complex homolog."
Proc. Natl. Acad. Sci. U.S.A. 94:4626-4630(1997) [PubMed] [Europe PMC] [Abstract]

"Hydrophobic ligand binding by Zn-alpha 2-glycoprotein, a soluble fat-depleting factor related to major histocompatibility complex proteins."
J. Biol. Chem. 276:35008-35013(2001) [PubMed] [Europe PMC] [Abstract]

"A proteomic analysis of human bile."
Mol. Cell. Proteomics 3:715-728(2004) [PubMed] [Europe PMC] [Abstract]

"Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."
Proteomics 4:454-465(2004) [PubMed] [Europe PMC] [Abstract]

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]

"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

"A strategy for precise and large scale identification of core fucosylated glycoproteins."
Mol. Cell. Proteomics 8:913-923(2009) [PubMed] [Europe PMC] [Abstract]

"Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules."
Science 283:1914-1919(1999) [PubMed] [Europe PMC] [Abstract]

"Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein."
J. Struct. Biol. 148:205-213(2004) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma."
J. Mol. Biol. 384:663-672(2008) [PubMed] [Europe PMC] [Abstract]