R-Spondin 2 (RSPO2)
Roof plate-specific spondin-2
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1) signaling. Xenopus and human RSPO2 enhanced mouse Wnt3a signaling in transfected human embryonic kidney cells. C-terminally truncated Xenopus Rspo2, but not the full-length protein, was secreted from cells and retained its activity. Mutation analysis showed that the TSP1 domain of Xenopus Rspo2 was dispensable for activity, but the furin domains were required. RT-PCR analysis showed that Xenopus embryos injected with Wnt8 or beta-catenin induced Rspo2 and Rspo3. Overexpression of Rspo2 in Xenopus activated the Wnt/beta-catenin pathway upstream of dishevelled (DVL1), interfered with Tgfb-type growth factors, and promoted myogenesis.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPM227Hu01 | Recombinant R-Spondin 2 (RSPO2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAM227Hu01 | Monoclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IP. |
| PAM227Hu01 | Polyclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLM227Hu01 | CLIA Kit for R-Spondin 2 (RSPO2) | CLIA assay for Antigen Detection |
| ELM227Hu01 | ELISA Kit for R-Spondin 2 (RSPO2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPM227Mu01 | Recombinant R-Spondin 2 (RSPO2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAM227Mu01 | Monoclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IP. |
| PAM227Mu01 | Polyclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLM227Mu01 | CLIA Kit for R-Spondin 2 (RSPO2) | CLIA assay for Antigen Detection |
| ELM227Mu01 | ELISA Kit for R-Spondin 2 (RSPO2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPM227Ra01 | Recombinant R-Spondin 2 (RSPO2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAM227Ra01 | Monoclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IP. |
| PAM227Ra01 | Polyclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLM227Ra01 | CLIA Kit for R-Spondin 2 (RSPO2) | CLIA assay for Antigen Detection |
| ELM227Ra01 | ELISA Kit for R-Spondin 2 (RSPO2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPM227Ca01 | Recombinant R-Spondin 2 (RSPO2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAM227Ca01 | Monoclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IP. |
| PAM227Ca01 | Polyclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLM227Ca01 | CLIA Kit for R-Spondin 2 (RSPO2) | CLIA assay for Antigen Detection |
| ELM227Ca01 | ELISA Kit for R-Spondin 2 (RSPO2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPM227Po01 | Recombinant R-Spondin 2 (RSPO2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAM227Po01 | Monoclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IP. |
| PAM227Po01 | Polyclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLM227Po01 | CLIA Kit for R-Spondin 2 (RSPO2) | CLIA assay for Antigen Detection |
| ELM227Po01 | ELISA Kit for R-Spondin 2 (RSPO2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPM227Bo01 | Recombinant R-Spondin 2 (RSPO2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAM227Bo01 | Monoclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IP. |
| PAM227Bo01 | Polyclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLM227Bo01 | CLIA Kit for R-Spondin 2 (RSPO2) | CLIA assay for Antigen Detection |
| ELM227Bo01 | ELISA Kit for R-Spondin 2 (RSPO2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPM227Rb01 | Recombinant R-Spondin 2 (RSPO2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAM227Rb01 | Monoclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IP. |
| PAM227Rb01 | Polyclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLM227Rb01 | CLIA Kit for R-Spondin 2 (RSPO2) | CLIA assay for Antigen Detection |
| ELM227Rb01 | ELISA Kit for R-Spondin 2 (RSPO2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPM227Mo01 | Recombinant R-Spondin 2 (RSPO2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAM227Mo01 | Monoclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IP. |
| PAM227Mo01 | Polyclonal Antibody to R-Spondin 2 (RSPO2) | WB; IHC; ICC; IF. | |
| Assay Kits | CLM227Mo01 | CLIA Kit for R-Spondin 2 (RSPO2) | CLIA assay for Antigen Detection |
| ELM227Mo01 | ELISA Kit for R-Spondin 2 (RSPO2) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"DNA sequence and analysis of human chromosome 8."
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"R-spondin proteins: a novel link to beta-catenin activation."
Cell Cycle 5:23-26(2006) [PubMed] [Europe PMC] [Abstract]
"LGR4 and LGR5 are R-spondin receptors mediating Wnt/beta-catenin and Wnt/PCP signalling."
EMBO Rep. 12:1055-1061(2011) [PubMed] [Europe PMC] [Abstract]
"Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling."
Nature 476:293-297(2011) [PubMed] [Europe PMC] [Abstract]
"LGR6 is a high affinity receptor of R-spondins and potentially functions as a tumor suppressor."
PLoS ONE 7:E37137-E37137(2012) [PubMed] [Europe PMC] [Abstract]