Canopy 2 Homolog (CNPY2)

ZSIG9; Cnpy2; MSAP; TMEM4; Transmembrane Protein 4; MIR-interacting saposin-like protein; Putative secreted protein Zsig9

SPN	L779
HGNC	13529
MGI	1928477
PubMed	22378787
RGD	1589072
UniProt	Q9Y2B0

TMEM4 encodes a predicted 182-amino acid type II transmembrane protein.The first 3 to 20 amino acids of MSAP constitute the SA region, which is followed by a saposin -like domain interrupted by 2 stretches of 30 and 40 amino acids.

Northern blot analysis detected MSAP expression in all adult and fetal tissues examined, with highest levels in adult placenta, liver, and pancreas. Western blot analysis of placenta and brain detected MSAP at an apparent molecular mass of 21 kD. Immunofluorescence microscopy showed colocalization of Msap with Mir in COS-7 cells. In cultured rodent primary hippocampal neurons, the 2 proteins localized around the nucleus and extended into the neurites.

Reference

"Selection of cDNAs encoding putative type II membrane proteins on the cell surface from a human full-length cDNA bank."
Gene 228:161-167(1999) [PubMed] [Europe PMC] [Abstract]

"MSAP is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain."
J. Biol. Chem. 278:35412-35420(2003) [PubMed] [Europe PMC] [Abstract]

"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]