PCNA Associated Factor 15kDa (PAF)

p15(PAF); L5; OEATC1; NS5ATP9; Overexpressed In Anaplastic Thyroid Carcinoma 1; Hepatitis C virus NS5A-transactivated protein 9

SPN	L322
HGNC	28961
MGI	1915276
PubMed	11313979
RGD	1303041
UniProt	Q15004

p15(PAF) bound endogenous PCNA in human embryonic kidney 293 cells, and mutation analysis indicated that ile65 and phe68 of p15(PAF) were required for the interaction. Overexpressed p15(PAF) competed with p21(WAF1) (CDKN1A) for PCNA binding in 293 cells. Expression of p15(PAF) was significantly increased in several types of tumor tissues, particularly in esophageal tumors. The deduced protein contains 111 amino acids. Northern blot analysis detected high expression in thymus and colon, intermediate expression in placenta, liver, kidney, spleen, ovary, small intestine, and peripheral blood leukocytes, weak expression in lung, pancreas, and testis, and no expression in heart, brain, skeletal muscle, and prostate. Expression was also detected in HeLa cells.

Reference

"Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer."
Oncogene 23:7734-7745(2004) [PubMed] [Europe PMC] [Abstract]

"KIAA0101 is overexpressed, and promotes growth and invasion in adrenal cancer."
PLoS ONE 6:E26866-E26866(2011) [PubMed] [Europe PMC] [Abstract]

"Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1."
DNA Res. 2:37-43(1995) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Analysis of the DNA sequence and duplication history of human chromosome 15."
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"p15(PAF), a novel PCNA associated factor with increased expression in tumor tissues."
Oncogene 20:484-489(2001) [PubMed] [Europe PMC] [Abstract]

"Overexpressed in anaplastic thyroid carcinoma-1 (OEATC-1) as a novel gene responsible for anaplastic thyroid carcinoma."
Cancer 103:1785-1790(2005) [PubMed] [Europe PMC] [Abstract]

"The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b."
Exp. Cell Res. 312:73-85(2006) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"ATF3 and p15PAF are novel gatekeepers of genomic integrity upon UV stress."
Cell Death Differ. 16:728-737(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"KIAA0101 interacts with BRCA1 and regulates centrosome number."
Mol. Cancer Res. 9:1091-1099(2011) [PubMed] [Europe PMC] [Abstract]

"Regulation of ubiquitin chain initiation to control the timing of substrate degradation."
Mol. Cell 42:744-757(2011) [PubMed] [Europe PMC] [Abstract]

"Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyclosome substrate."
Proc. Natl. Acad. Sci. U.S.A. 108:9845-9850(2011) [PubMed] [Europe PMC] [Abstract]

"Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass."
Nat. Cell Biol. 14:1089-1098(2012) [PubMed] [Europe PMC] [Abstract]