Protease, Serine 50 (PRSS50)

TSP50; CT20; Cancer/Testis Antigen 20; Testes Specific Protease 50; Testis-specific protease-like protein 50

SPN	L149
HGNC	17910
MGI	2447303
PubMed	24799889
UniProt	Q9UI38

TSP50 DNA methylation correlated with lack of TSP50 expression in bladder, blood, breast, colon, lung, kidney, placenta, and ovary tissues, while demethylation correlated with its expression in testis. TSP50 was both abnormally hypomethylated and expressed in some breast and ovarian cancers, but not in the corresponding normal tissues.

While TSP50 shares significant identity with several serine proteases, the catalytic serine is replaced by threonine. Immunohistochemical analysis showed TSP50 expressed in spermatocytes, but not in mature sperm. Western blot analysis detected TSP50 in 92% of breast cancer samples tested, but not in normal matched tissues. In situ hybridization confirmed expression of TSP50 in malignant breast cancer cells.

Reference

"Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer."
Cancer Res. 59:3215-3221(1999) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"TSP50 encodes a testis-specific protease and is negatively regulated by p53."
Cancer Res. 67:1239-1245(2007) [PubMed] [Europe PMC] [Abstract]