Pim-3 Oncogene (PIM3)

Serine/threonine-protein kinase pim-3

SPN	F326
HGNC	19310
MGI	1355297
PubMed	12748291
UniProt	Q86V86

The deduced 326-amino acid protein has a calculated molecular mass of 35.9 kD. PIM3 shares 95% amino acid identity with rodent Pim3 and 57.1% and 44.0% amino acid identity with human PIM1 and PIM2 , respectively.

Northern blot analysis detected a 2.4-kb transcript in heart, skeletal muscle, brain, spleen, kidney, placenta, lung, and peripheral blood leukocytes. Expression was not detected in colon, thymus, liver, and small intestine. Immunohistochemical analysis showed no expression of PIM3 in normal liver, but it localized diffusely in large regenerative nodules and adenomatous hyperplasia, lesions with precancerous potential, adjacent to hepatocellar carcinoma areas. PIM3 was expressed in all hepatoma cell lines examined.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPF326Hu01	Recombinant Pim-3 Oncogene (PIM3)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAF326Hu01	Monoclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IP.
Antibodies	PAF326Hu01	Polyclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IF.
Assay Kits	CLF326Hu01	CLIA Kit for Pim-3 Oncogene (PIM3)	CLIA assay for Antigen Detection
Assay Kits	ELF326Hu01	ELISA Kit for Pim-3 Oncogene (PIM3)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPF326Mu01	Recombinant Pim-3 Oncogene (PIM3)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAF326Mu01	Monoclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IP.
Antibodies	PAF326Mu01	Polyclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IF.
Assay Kits	CLF326Mu01	CLIA Kit for Pim-3 Oncogene (PIM3)	CLIA assay for Antigen Detection
Assay Kits	ELF326Mu01	ELISA Kit for Pim-3 Oncogene (PIM3)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPF326Ra01	Recombinant Pim-3 Oncogene (PIM3)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAF326Ra01	Monoclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IP.
Antibodies	PAF326Ra01	Polyclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IF.
Assay Kits	CLF326Ra01	CLIA Kit for Pim-3 Oncogene (PIM3)	CLIA assay for Antigen Detection
Assay Kits	ELF326Ra01	ELISA Kit for Pim-3 Oncogene (PIM3)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPF326Ca01	Recombinant Pim-3 Oncogene (PIM3)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAF326Ca01	Monoclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IP.
Antibodies	PAF326Ca01	Polyclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IF.
Assay Kits	CLF326Ca01	CLIA Kit for Pim-3 Oncogene (PIM3)	CLIA assay for Antigen Detection
Assay Kits	ELF326Ca01	ELISA Kit for Pim-3 Oncogene (PIM3)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPF326Po01	Recombinant Pim-3 Oncogene (PIM3)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAF326Po01	Monoclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IP.
Antibodies	PAF326Po01	Polyclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IF.
Assay Kits	CLF326Po01	CLIA Kit for Pim-3 Oncogene (PIM3)	CLIA assay for Antigen Detection
Assay Kits	ELF326Po01	ELISA Kit for Pim-3 Oncogene (PIM3)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPF326Bo01	Recombinant Pim-3 Oncogene (PIM3)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAF326Bo01	Monoclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IP.
Antibodies	PAF326Bo01	Polyclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IF.
Assay Kits	CLF326Bo01	CLIA Kit for Pim-3 Oncogene (PIM3)	CLIA assay for Antigen Detection
Assay Kits	ELF326Bo01	ELISA Kit for Pim-3 Oncogene (PIM3)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPF326Rb01	Recombinant Pim-3 Oncogene (PIM3)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAF326Rb01	Monoclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IP.
Antibodies	PAF326Rb01	Polyclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IF.
Assay Kits	CLF326Rb01	CLIA Kit for Pim-3 Oncogene (PIM3)	CLIA assay for Antigen Detection
Assay Kits	ELF326Rb01	ELISA Kit for Pim-3 Oncogene (PIM3)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPF326Mo01	Recombinant Pim-3 Oncogene (PIM3)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAF326Mo01	Monoclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IP.
Antibodies	PAF326Mo01	Polyclonal Antibody to Pim-3 Oncogene (PIM3)	WB; IHC; ICC; IF.
Assay Kits	CLF326Mo01	CLIA Kit for Pim-3 Oncogene (PIM3)	CLIA assay for Antigen Detection
Assay Kits	ELF326Mo01	ELISA Kit for Pim-3 Oncogene (PIM3)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Aberrant expression of serine/threonine kinase Pim-3 in hepatocellular carcinoma development and its role in the proliferation of human hepatoma cell lines."
Int. J. Cancer 114:209-218(2005) [PubMed] [Europe PMC] [Abstract]

"The transcriptional landscape of the mammalian genome."
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence of human chromosome 22."
Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Consistency checks for characterizing protein forms."
Comput. Biol. Chem. 27:29-35(2003) [PubMed] [Europe PMC] [Abstract]

"Protein phosphatase 2A regulates the stability of Pim protein kinases."
J. Biol. Chem. 278:4800-4805(2003) [PubMed] [Europe PMC] [Abstract]

"Pim-3, a proto-oncogene with serine/threonine kinase activity, is aberrantly expressed in human pancreatic cancer and phosphorylates bad to block bad-mediated apoptosis in human pancreatic cancer cell lines."
Cancer Res. 66:6741-6747(2006) [PubMed] [Europe PMC] [Abstract]

"Proto-oncogene, Pim-3 with serine/threonine kinase activity, is aberrantly expressed in human colon cancer cells and can prevent Bad-mediated apoptosis."
Cancer Sci. 98:321-328(2007) [PubMed] [Europe PMC] [Abstract]

"Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels."
Cancer Res. 68:5076-5085(2008) [PubMed] [Europe PMC] [Abstract]

"Aberrant Pim-3 expression is involved in gastric adenoma-adenocarcinoma sequence and cancer progression."
J. Cancer Res. Clin. Oncol. 134:481-488(2008) [PubMed] [Europe PMC] [Abstract]